Any feedback?
Literature summary extracted from
- Functional expression of a putative geraniol 8-hydroxylase by reconstitution of bacterially expressed plant CYP76F45 and NADPH-cytochrome P450 reductase CPR I from Croton stellatopilosus Ohba (2015), Phytochemistry, 118, 204-215 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.83 | gene CsCPR I, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis and tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3). Only simultaneous incubation of the membrane-bound enzymes CsCYP76F45 and CsCPR I with the substrate geraniol and the coenzyme NADPH allows 8-hydroxygeraniol formation | Croton stellatopilosus |
| 1.14.14.83 | gene CYP76F45, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis and tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3). Only simultaneous incubation of the membrane-bound enzymes CsCYP76F45 and CsCPR I with the substrate geraniol and the coenzyme NADPH allows 8-hydroxygeraniol formation | Croton stellatopilosus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.83 | membrane | membrane-bound | Croton stellatopilosus | 16020 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.83 | geraniol + [reduced NADPH-hemoprotein reductase] + O2 | Croton stellatopilosus | - |
(6E)-8-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |  |
| 1.14.14.83 | geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2 | Croton stellatopilosus | - |
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.83 | geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2 | Croton stellatopilosus Ohba | - |
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.6.2.4 | Croton stellatopilosus | - |
- |
- |
| 1.14.14.83 | Croton stellatopilosus | A0A0A7DLM4 | - |
- |
| 1.14.14.83 | Croton stellatopilosus | A0A0A8KYK4 | cf. EC 1.6.2.4 | - |
| 1.14.14.83 | Croton stellatopilosus Ohba | A0A0A7DLM4 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.6.2.4 | leaf | - |
Croton stellatopilosus | - |
| 1.6.2.4 | shoot | - |
Croton stellatopilosus | - |
| 1.6.2.4 | twig | - |
Croton stellatopilosus | - |
| 1.14.14.83 | leaf | - |
Croton stellatopilosus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.83 | farnesol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Croton stellatopilosus | 12-hydroxyfarnesol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.83 | farnesol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Croton stellatopilosus Ohba | 12-hydroxyfarnesol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.83 | geraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Croton stellatopilosus | (6E)-8-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.83 | geraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Croton stellatopilosus Ohba | (6E)-8-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.83 | geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Croton stellatopilosus | 18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.83 | geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Croton stellatopilosus Ohba | 18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.83 | geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Croton stellatopilosus | 16-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.83 | geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Croton stellatopilosus Ohba | 16-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.6.2.4 | ? | x * 79000, calculated from amino acid sequence | Croton stellatopilosus |
| 1.14.14.83 | ? | x * 56700, about, sequence calculation | Croton stellatopilosus |
| 1.14.14.83 | ? | x * 56700, calculated, Cyp76F45 | Croton stellatopilosus |
| 1.14.14.83 | ? | x * 78700, about, sequence calculation, x * 96000, recombinant His-tagged enzyme, SDS-PAGE | Croton stellatopilosus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.6.2.4 | CPR I | - |
Croton stellatopilosus |
| 1.6.2.4 | NADPH-cytochrome P450 reductase | - |
Croton stellatopilosus |
| 1.14.14.83 | CPR I | - |
Croton stellatopilosus |
| 1.14.14.83 | CYP76F45 | - |
Croton stellatopilosus |
| 1.14.14.83 | G8H | - |
Croton stellatopilosus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.83 | cytochrome P450 | a cytochrome P450 monooxygenase | Croton stellatopilosus | |
| 1.14.14.83 | cytochrome P450 | an NADPH-cytochrome P450 reductase | Croton stellatopilosus | |
| 1.14.14.83 | FAD | - |
Croton stellatopilosus | |
| 1.14.14.83 | FMN | - |
Croton stellatopilosus | |
| 1.14.14.83 | heme | - |
Croton stellatopilosus | |
| 1.14.14.83 | NADPH | an NADPH-cytochrome P450 reductase | Croton stellatopilosus | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.14.14.83 | Croton stellatopilosus | sequence calculation | - |
5.27 |
| 1.14.14.83 | Croton stellatopilosus | calculated, Cyp76F45 | - |
8.6 |
| 1.14.14.83 | Croton stellatopilosus | sequence calculation | - |
8.63 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.6.2.4 | physiological function | CYP76F45 and NADPH-cytochrome P450 reductase CPR I function as the enzyme geraniol-8-hydroxylase, which is likely to be involved in the biosynthesis of the indole alkaloid in Croton stellatopilosus | Croton stellatopilosus |
| 1.14.14.83 | evolution | enzyme CsCPR I belongs to Class I of dicotyledonous CPRs | Croton stellatopilosus |
| 1.14.14.83 | evolution | enzyme CYP76F45 belongs to the CYP76 family of dicotyledonous CPR class I | Croton stellatopilosus |
| 1.14.14.83 | additional information | the enzyme contains specific oxygen- and heme-binding sites | Croton stellatopilosus |
| 1.14.14.83 | additional information | the enzyme contains two FMN-, FAD-, and NADPH-binding sites and one P450-binding site | Croton stellatopilosus |
| 1.14.14.83 | physiological function | enzymes CYP97C27 and CPR I function as the enzyme geraniol-8-hydroxylase (G8H), which is likely to be involved in the biosynthesis of the indole alkaloid 19-E-vallesamine in Croton stellatopilosus | Croton stellatopilosus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
