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Literature summary extracted from

  • Henquet, M.G.L.; Prota, N.; van der Hooft, J.J.J.; Varbanova-Herde, M.; Hulzink, R.J.M.; de Vos, M.; Prins, M.; de Both, M.T.J.; Franssen, M.C.R.; Bouwmeester, H.; Jongsma, M.
    Identification of a drimenol synthase and drimenol oxidase from Persicaria hydropiper, involved in the biosynthesis of insect deterrent drimanes (2017), Plant J., 90, 1052-1063 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.72 0.011
-
 drimenol pH 7.5, 25°C Persicaria hydropiper

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.194 Mg2+ presence of two DDxxD motifs in the sequence Persicaria hydropiper

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.72 Persicaria hydropiper W0FKI0
-
-
4.2.3.194 Persicaria hydropiper W0FFD7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.72 drimenol + [reduced NADPH-hemoprotein reductase] + O2
-
 Persicaria hydropiper drimendiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.72 additional information DOX1 catalyses the conversion from drimenol to drimendiol, and not the further oxidation into an aldehyde Persicaria hydropiper ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.72 ? x * 56149, calculated Persicaria hydropiper
4.2.3.194 ? x * 64729, calculated Persicaria hydropiper

Synonyms

EC Number Synonyms Comment Organism
1.14.14.72 CYP76AJ1
-
 Persicaria hydropiper
1.14.14.72 DOX1
-
 Persicaria hydropiper
1.14.14.72 drimenol oxidase 1
-
 Persicaria hydropiper

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.72 cytochrome P450
-
 Persicaria hydropiper

General Information

EC Number General Information Comment Organism
1.14.14.72 physiological function coexpression of drimenol synthase with drimenol oxidase DOX1 in yeast yields drimendiol, the 12-hydroxylation product of drimenol, as a major product, and cinnamolide. Upon coexpression of drimenol synthase with drimenol oxidase DOX1 in Nicotiana benthamiana leaves, drimenol is almost completely converted into cinnamolide and several additional drimenol derivatives are observed Persicaria hydropiper
4.2.3.194 physiological function expression in yeast and plants results in production of drimenol alone. Coexpression of PhDS with DOX1 in yeast yields drimendiol, the 12-hydroxylation product of drimenol, as a major product, and cinnamolide. By transient expression of drimenol synthase and DOX1 in Nicotiana benthamiana leaves, drimenol is almost completely converted into cinnamolide and several additional drimenol derivatives Persicaria hydropiper