BRENDA - Enzyme Database

Identification of a drimenol synthase and drimenol oxidase from Persicaria hydropiper, involved in the biosynthesis of insect deterrent drimanes

Henquet, M.G.L.; Prota, N.; van der Hooft, J.J.J.; Varbanova-Herde, M.; Hulzink, R.J.M.; de Vos, M.; Prins, M.; de Both, M.T.J.; Franssen, M.C.R.; Bouwmeester, H.; Jongsma, M.; Plant J. 90, 1052-1063 (2017)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.72
0.011
-
drimenol
pH 7.5, 25°C
Persicaria hydropiper
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.3.194
Mg2+
presence of two DDxxD motifs in the sequence
Persicaria hydropiper
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.72
Persicaria hydropiper
W0FKI0
-
-
4.2.3.194
Persicaria hydropiper
W0FFD7
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.72
drimenol + [reduced NADPH-hemoprotein reductase] + O2
-
743475
Persicaria hydropiper
drimendiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.72
additional information
DOX1 catalyses the conversion from drimenol to drimendiol, and not the further oxidation into an aldehyde
743475
Persicaria hydropiper
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.14.14.72
?
x * 56149, calculated
Persicaria hydropiper
4.2.3.194
?
x * 64729, calculated
Persicaria hydropiper
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.72
cytochrome P450
-
Persicaria hydropiper
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.72
cytochrome P450
-
Persicaria hydropiper
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.72
0.011
-
drimenol
pH 7.5, 25°C
Persicaria hydropiper
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.3.194
Mg2+
presence of two DDxxD motifs in the sequence
Persicaria hydropiper
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.72
drimenol + [reduced NADPH-hemoprotein reductase] + O2
-
743475
Persicaria hydropiper
drimendiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.72
additional information
DOX1 catalyses the conversion from drimenol to drimendiol, and not the further oxidation into an aldehyde
743475
Persicaria hydropiper
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.14.72
?
x * 56149, calculated
Persicaria hydropiper
4.2.3.194
?
x * 64729, calculated
Persicaria hydropiper
General Information
EC Number
General Information
Commentary
Organism
1.14.14.72
physiological function
coexpression of drimenol synthase with drimenol oxidase DOX1 in yeast yields drimendiol, the 12-hydroxylation product of drimenol, as a major product, and cinnamolide. Upon coexpression of drimenol synthase with drimenol oxidase DOX1 in Nicotiana benthamiana leaves, drimenol is almost completely converted into cinnamolide and several additional drimenol derivatives are observed
Persicaria hydropiper
4.2.3.194
physiological function
expression in yeast and plants results in production of drimenol alone. Coexpression of PhDS with DOX1 in yeast yields drimendiol, the 12-hydroxylation product of drimenol, as a major product, and cinnamolide. By transient expression of drimenol synthase and DOX1 in Nicotiana benthamiana leaves, drimenol is almost completely converted into cinnamolide and several additional drimenol derivatives
Persicaria hydropiper
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.14.72
physiological function
coexpression of drimenol synthase with drimenol oxidase DOX1 in yeast yields drimendiol, the 12-hydroxylation product of drimenol, as a major product, and cinnamolide. Upon coexpression of drimenol synthase with drimenol oxidase DOX1 in Nicotiana benthamiana leaves, drimenol is almost completely converted into cinnamolide and several additional drimenol derivatives are observed
Persicaria hydropiper
4.2.3.194
physiological function
expression in yeast and plants results in production of drimenol alone. Coexpression of PhDS with DOX1 in yeast yields drimendiol, the 12-hydroxylation product of drimenol, as a major product, and cinnamolide. By transient expression of drimenol synthase and DOX1 in Nicotiana benthamiana leaves, drimenol is almost completely converted into cinnamolide and several additional drimenol derivatives
Persicaria hydropiper