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Literature summary extracted from

  • Cui, G.; Duan, L.; Jin, B.; Qian, J.; Xue, Z.; Shen, G.; Snyder, J.H.; Song, J.; Chen, S.; Huang, L.; Peters, R.J.; Qi, X.
    Functional divergence of diterpene syntheses in the medicinal plant Salvia miltiorrhiza (2015), Plant Physiol., 169, 1607-1618 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.2.1.173 synthesis CPS4 and kaurene synthase-like2 act together to produce ent-13-epi-manoyl oxide Salvia miltiorrhiza

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.173
-
Salvia miltiorrhiza
4.2.3.186
-
Salvia miltiorrhiza f. alba
5.5.1.12 gene SmCPS1, phylogenetic analysis, a 289-bp gene-specific sequence including the 3' UTR of SmCPS1 is amplified by PCR using cDNA as a template and then cloned into the pK7GWIWG (II) binary, then introduced into Agrobacterium tumefaciens strain EHA105 by electroporation for ransformation of leaf explants from Salvia miltiorrhiza bh2-7 plants, recombinant expression of His-tagged enzyme in Escherichia coli strains Tuner (DE3) or Origami B (DE3) Salvia miltiorrhiza f. alba
5.5.1.12 gene SmCPS2, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strains Tuner (DE3) or Origami B (DE3) Salvia miltiorrhiza f. alba

Protein Variants

EC Number Protein Variants Comment Organism
5.5.1.12 additional information knockdown of SmCPS1 by RNAi Salvia miltiorrhiza f. alba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.12 geranylgeranyl diphosphate Salvia miltiorrhiza f. alba
-
(+)-copalyl diphosphate
-
r
5.5.1.12 geranylgeranyl diphosphate Salvia miltiorrhiza f. alba bh2-7
-
(+)-copalyl diphosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.173 Salvia miltiorrhiza A0A0U2D9C5 f. alba
-
4.2.3.186 Salvia miltiorrhiza f. alba A0A0A7ANA5
-
-
5.5.1.12 Salvia miltiorrhiza f. alba A0A0A7AN31
-
-
5.5.1.12 Salvia miltiorrhiza f. alba A0A0A7ANT1
-
-
5.5.1.12 Salvia miltiorrhiza f. alba bh2-7 A0A0A7AN31
-
-
5.5.1.12 Salvia miltiorrhiza f. alba bh2-7 A0A0A7ANT1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.12 recombinant His-tagged enzyme by nickel affinity chromatography from Escherichia coli strains Tuner (DE3) or Origami B (DE3) Salvia miltiorrhiza f. alba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.173 sepal most highly expressed Salvia miltiorrhiza
-
4.2.3.186 additional information expressed throughout all tissues of adult plants Salvia miltiorrhiza f. alba
-
4.2.3.186 root most highly expressed in root xylem Salvia miltiorrhiza f. alba
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.173 geranylgeranyl diphosphate + H2O
-
Salvia miltiorrhiza ent-8alpha-hydroxylabd-13-en-15-yl diphosphate
-
?
4.2.3.186 ent-8alpha-hydroxylabd-13-en-15-yl diphosphate
-
Salvia miltiorrhiza f. alba ent-13-epi-manoyl oxide + diphosphate
-
?
4.2.3.186 additional information in presence of geranylgeranyl diphosphate, combining copalyl diphosphate synthase CPS4 and KSL2 leads to production of ent-13-epi-manoyl oxide. No product is observed when CPS1, CPS2 or CPS3 is combined with KSL2 Salvia miltiorrhiza f. alba ?
-
?
5.5.1.12 geranylgeranyl diphosphate
-
Salvia miltiorrhiza f. alba (+)-copalyl diphosphate
-
r
5.5.1.12 geranylgeranyl diphosphate
-
Salvia miltiorrhiza f. alba bh2-7 (+)-copalyl diphosphate
-
r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.173 copal-8-ol diphosphate synthase
-
Salvia miltiorrhiza
4.2.1.173 CPS4
-
Salvia miltiorrhiza
4.2.3.186 KSL2
-
Salvia miltiorrhiza f. alba
5.5.1.12 CPS1
-
Salvia miltiorrhiza f. alba
5.5.1.12 CPS2
-
Salvia miltiorrhiza f. alba
5.5.1.12 SmCPS1
-
Salvia miltiorrhiza f. alba
5.5.1.12 SmCPS2
-
Salvia miltiorrhiza f. alba

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.5.1.12 25
-
assay at Salvia miltiorrhiza f. alba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5.1.12 8
-
assay at Salvia miltiorrhiza f. alba

General Information

EC Number General Information Comment Organism
4.2.3.186 physiological function KSL2 may have dual function both in gibberlinic acid and in diterpenoid biosynthesis Salvia miltiorrhiza f. alba
5.5.1.12 evolution the enzyme belongs to the diterpene synthase family Salvia miltiorrhiza f. alba
5.5.1.12 additional information metabolomic profiling, and homology modeling and molecular docking Salvia miltiorrhiza f. alba