BRENDA - Enzyme Database show

Completion of the seven-step pathway from tabersonine to the anticancer drug precursor vindoline and its assembly in yeast

Qu, Y.; Easson, M.L.; Froese, J.; Simionescu, R.; Hudlicky, T.; De Luca, V.; Proc. Natl. Acad. Sci. USA 112, 6224-6229 (2015)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.99.41
16-methoxytabersonine + NADPH + H+
Catharanthus roseus
-
3-hydroxy-16-methoxy-2,3-dihydrotabersonine
-
-
?
1.1.99.41
tabersonine + NADPH + H+
Catharanthus roseus
-
2,3-dihydro-3-hydroxytabersonine + NADP+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.99.41
Catharanthus roseus
A0A161CAI1
cultivar Little Delicata
-
1.14.14.50
Catharanthus roseus
I1TEM1
cultivar Little Delicata
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.99.41
epidermis
-
Catharanthus roseus
-
1.1.99.41
leaf
-
Catharanthus roseus
-
1.14.14.50
epidermis
-
Catharanthus roseus
-
1.14.14.50
leaf
-
Catharanthus roseus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.99.41
16-hydroxytabersonine + NADPH + H+
-
743687
Catharanthus roseus
?
-
-
-
?
1.1.99.41
16-methoxytabersonine + NADPH + H+
-
743687
Catharanthus roseus
3-hydroxy-16-methoxy-2,3-dihydrotabersonine
-
-
-
?
1.1.99.41
2,3-dihydrotabersonine + NADPH + H+
-
743687
Catharanthus roseus
?
-
-
-
?
1.1.99.41
tabersonine + NADPH + H+
-
743687
Catharanthus roseus
2,3-dihydro-3-hydroxytabersonine + NADP+
-
-
-
?
1.14.14.50
16-methoxytabersonine + [reduced NADPH-hemoprotein reductase] + O2
-
743687
Catharanthus roseus
(3R)-3-hydroxy-16-methoxy-1,2-didehydro-2,3-dihydrotabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.50
tabersonine + [reduced NADPH-hemoprotein reductase] + O2
-
743687
Catharanthus roseus
(3R)-3-hydroxy-1,2-didehydro-2,3-dihydrotabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.99.41
16-methoxytabersonine + NADPH + H+
Catharanthus roseus
-
3-hydroxy-16-methoxy-2,3-dihydrotabersonine
-
-
?
1.1.99.41
tabersonine + NADPH + H+
Catharanthus roseus
-
2,3-dihydro-3-hydroxytabersonine + NADP+
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.99.41
epidermis
-
Catharanthus roseus
-
1.1.99.41
leaf
-
Catharanthus roseus
-
1.14.14.50
epidermis
-
Catharanthus roseus
-
1.14.14.50
leaf
-
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.99.41
16-hydroxytabersonine + NADPH + H+
-
743687
Catharanthus roseus
?
-
-
-
?
1.1.99.41
16-methoxytabersonine + NADPH + H+
-
743687
Catharanthus roseus
3-hydroxy-16-methoxy-2,3-dihydrotabersonine
-
-
-
?
1.1.99.41
2,3-dihydrotabersonine + NADPH + H+
-
743687
Catharanthus roseus
?
-
-
-
?
1.1.99.41
tabersonine + NADPH + H+
-
743687
Catharanthus roseus
2,3-dihydro-3-hydroxytabersonine + NADP+
-
-
-
?
1.14.14.50
16-methoxytabersonine + [reduced NADPH-hemoprotein reductase] + O2
-
743687
Catharanthus roseus
(3R)-3-hydroxy-16-methoxy-1,2-didehydro-2,3-dihydrotabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.50
tabersonine + [reduced NADPH-hemoprotein reductase] + O2
-
743687
Catharanthus roseus
(3R)-3-hydroxy-1,2-didehydro-2,3-dihydrotabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?