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Literature summary extracted from

  • Srisook, T.; Vongsetskul, T.; Sucharitakul, J.; Chaiyen, P.; Tangboriboonrat, P.
    Immobilization of 3-hydroxybenzoate 6-hydroxylase onto functionalized electrospun polycaprolactone ultrafine fibers A novel heterogeneous catalyst (2014), React. Funct. Polym., 82, 41-46 .
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.14.13.24 immobilized onto surface-functionalized electrospun polycaprolactone (PCL) fibers can tolerate the changes in temperature and pH better than the free enzyme can. The immobilized enzyme can be reused at least 10 times with more than 60% of the original activity at pH 8 and 25°C. The 3-hydroxybenzoate 6-hydroxylase fibers are potentially useful as a heterogeneous catalyst under the conditions in which free enzyme can not endure. Rhodococcus jostii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.24 0.046
-
 3-hydroxybenzoate pH 8, 25°C, native enzyme Rhodococcus jostii
1.14.13.24 0.142
-
 3-hydroxybenzoate pH 8, 25°C, enzyme immobilized onto surface-functionalized electrospun polycaprolactone (PCL) fibers Rhodococcus jostii
1.14.13.24 2.5
-
 NADH pH 8, 25°C, native enzyme Rhodococcus jostii

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.24 Rhodococcus jostii Q0SFK6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.24 3-hydroxybenzoate + NADH + H+ + O2
-
 Rhodococcus jostii 2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.24 3HB6H
-
 Rhodococcus jostii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.24 0.0178
-
 3-hydroxybenzoate pH 8, 25°C, enzyme immobilized onto surface-functionalized electrospun polycaprolactone (PCL) fibers Rhodococcus jostii
1.14.13.24 21
-
 3-hydroxybenzoate pH 8, 25°C, native enzyme Rhodococcus jostii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.24 7
-
 soluble enzyme Rhodococcus jostii
1.14.13.24 8
-
 enzyme immobilized onto surface-functionalized electrospun polycaprolactone (PCL) fibers Rhodococcus jostii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.24 5.5 8 at pH 5.5-8, the residual activity of soluble enzyme is less than that of the immobilized one Rhodococcus jostii
1.14.13.24 9 10 at pH 9 and 10, the enzyme activity of of the soluble enzyme is higher than that of the immobilized enzyme Rhodococcus jostii