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Literature summary extracted from
- Research journal of pharmaceutical, biological and chemical sciences purification, characterization, thermostability and shelf life studies of glucose oxidase from Aspergillus niger PIL7 (2015), Res. J. Pharm. Biol. Chem. Sci., 6, 1666-1678 .
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.1.3.4 | analysis | GOx is the main component in glucose biosensors for determination of glucose in industrial solutions and in body fluids such as blood and urine | Aspergillus niger |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.3.4 | Ag+ | - |
Aspergillus niger |  |
| 1.1.3.4 | Co2+ | - |
Aspergillus niger | |
| 1.1.3.4 | Hg2+ | - |
Aspergillus niger | |
| 1.1.3.4 | Ni2+ | - |
Aspergillus niger | |
| 1.1.3.4 | Pb2+ | - |
Aspergillus niger | |
| 1.1.3.4 | Zn2+ | - |
Aspergillus niger | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.3.4 | additional information | - |
additional information | enzyme thermodynamics and kinetics | Aspergillus niger | |
| 1.1.3.4 | 89.13 | - |
beta-D-glucose | pH 6.0, 37°C | Aspergillus niger | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.3.4 | CuCl2 | activates | Aspergillus niger | |
| 1.1.3.4 | KCN | activates | Aspergillus niger | |
| 1.1.3.4 | additional information | no effect by NaF on enzyme activity | Aspergillus niger |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 160000 | - |
gel filtration | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.3.4 | beta-D-glucose + O2 | Aspergillus niger | - |
D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.3.4 | beta-D-glucose + O2 | Aspergillus niger PIL7 | - |
D-glucono-1,5-lactone + H2O2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.3.4 | Aspergillus niger | - |
- |
- |
| 1.1.3.4 | Aspergillus niger PIL7 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.3.4 | native enzyme 9.5fold from strain PIL7 by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and gel filtration | Aspergillus niger |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 28.81 | - |
purified native enzyme, pH 6.0, 37°C | Aspergillus niger |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.3.4 | -20°C, purified enzyme, 6 months without addition of additives, complete stable, no loss of activity | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.3.4 | 2-deoxy-D-glucose + O2 | 10% activity compared to beta-D-glucose | Aspergillus niger | 2-deoxy-D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.3.4 | 2-deoxy-D-glucose + O2 | 10% activity compared to beta-D-glucose | Aspergillus niger PIL7 | 2-deoxy-D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.3.4 | 3,6-methyl-D-glucose + O2 | 10% activity compared to beta-D-glucose | Aspergillus niger | 3-O,6-O-dimethyl-D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.3.4 | 3,6-methyl-D-glucose + O2 | 10% activity compared to beta-D-glucose | Aspergillus niger PIL7 | 3-O,6-O-dimethyl-D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.3.4 | 4-deoxy-D-glucose + O2 | 7% activity compared to beta-D-glucose | Aspergillus niger | 4-deoxy-D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.3.4 | 4-deoxy-D-glucose + O2 | 7% activity compared to beta-D-glucose | Aspergillus niger PIL7 | 4-deoxy-D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.3.4 | 4-O-methyl-D-glucose + O2 | 8% activity compared to beta-D-glucose | Aspergillus niger | 4-O-methyl-D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.3.4 | 6-deoxy-D-glucose + O2 | 12% activity compared to beta-D-glucose | Aspergillus niger | 6-deoxy-D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.3.4 | beta-D-glucose + O2 | - |
Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.3.4 | beta-D-glucose + O2 | - |
Aspergillus niger PIL7 | D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.3.4 | D-mannose + O2 | 9% activity compared to beta-D-glucose | Aspergillus niger | ? + H2O2 | - |
? | |
| 1.1.3.4 | L-sorbose + O2 | 15% activity compared to beta-D-glucose | Aspergillus niger | ? + H2O2 | - |
? | |
| 1.1.3.4 | additional information | no activity with 2-deoxy-6-fluoro-D-glucose, 4,6-dimethyl-D-glucose, beta-deoxy-D-glucose, 6-O-methyl-D-glucose, D-glucono-delta-lactone, L-gulono-gamma-lactone, D-gulono-gamma-lactone, D-glucuronolactone, altrose, galactose, xylose, idose, cellobiose, D-kabinose, L-arabinose, or D-fructose | Aspergillus niger | ? | - |
? | |
| 1.1.3.4 | additional information | no activity with 2-deoxy-6-fluoro-D-glucose, 4,6-dimethyl-D-glucose, beta-deoxy-D-glucose, 6-O-methyl-D-glucose, D-glucono-delta-lactone, L-gulono-gamma-lactone, D-gulono-gamma-lactone, D-glucuronolactone, altrose, galactose, xylose, idose, cellobiose, D-kabinose, L-arabinose, or D-fructose | Aspergillus niger PIL7 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.3.4 | monomer | 1 * 160000, SDS-PAGE | Aspergillus niger |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.3.4 | GOX | - |
Aspergillus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 30 | - |
- |
Aspergillus niger |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 20 | 70 | activity range, profile overview. About 90% of maximal activity at 20°C, 90% at 55°C, rapid deactivation above | Aspergillus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | additional information | - |
thermal inactivation thermodynamic parameters of GOx, overview | Aspergillus niger |
| 1.1.3.4 | 30 | - |
purified enzyme, completely stable for at least 180 min | Aspergillus niger |
| 1.1.3.4 | 55 | - |
purified enzyme, retains 57 % activity after 180 min | Aspergillus niger |
| 1.1.3.4 | 65 | - |
purified enzyme, loss of 50% activity after 27 min | Aspergillus niger |
| 1.1.3.4 | 70 | - |
purified enzyme, inactivation | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 6 | - |
- |
Aspergillus niger |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 2.5 | 8.5 | activity range, profile overview. 85% of maximal activity at pH 5.0, 51% activity at pH 7.0 | Aspergillus niger |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.3.4 | FAD | - |
Aspergillus niger | |
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