Literature summary extracted from
Ambrose, K.V.; Tian, Z.; Wang, Y.; Smith, J.; Zylstra, G.; Huang, B.; Belanger, F.C.
Functional characterization of salicylate hydroxylase from the fungal endophyte Epichloe festucae (2015), Sci. Rep., 5, 10939 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.13.1 |
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells |
Epichloe festucae |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.13.1 |
Epichloe festucae |
A0A0A1E7Z2 |
isolate Rose City |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.13.1 |
TALON metal affinity resin column chromatography |
Epichloe festucae |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.13.1 |
2,5-dihydroxybenzoic acid + NADH + H+ + O2 |
146% activity compared to salicylate |
Epichloe festucae |
2,5-dihydroxyphenol + NAD+ + H2O + CO2 |
- |
? |
|
1.14.13.1 |
salicylate + NADH + H+ + O2 |
100% activity |
Epichloe festucae |
catechol + NAD+ + H2O + CO2 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.14.13.1 |
? |
x * 44000, SDS-PAGE |
Epichloe festucae |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.13.1 |
Efe-shyA |
- |
Epichloe festucae |
1.14.13.1 |
salicylate hydroxylase |
- |
Epichloe festucae |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.13.1 |
NADH |
- |
Epichloe festucae |
|