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Literature summary extracted from

  • Ambrose, K.V.; Tian, Z.; Wang, Y.; Smith, J.; Zylstra, G.; Huang, B.; Belanger, F.C.
    Functional characterization of salicylate hydroxylase from the fungal endophyte Epichloe festucae (2015), Sci. Rep., 5, 10939 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.1 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells Epichloe festucae

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.1 Epichloe festucae A0A0A1E7Z2 isolate Rose City
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.1 TALON metal affinity resin column chromatography Epichloe festucae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.1 2,5-dihydroxybenzoic acid + NADH + H+ + O2 146% activity compared to salicylate Epichloe festucae 2,5-dihydroxyphenol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2 100% activity Epichloe festucae catechol + NAD+ + H2O + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.1 ? x * 44000, SDS-PAGE Epichloe festucae

Synonyms

EC Number Synonyms Comment Organism
1.14.13.1 Efe-shyA
-
 Epichloe festucae
1.14.13.1 salicylate hydroxylase
-
 Epichloe festucae

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.1 NADH
-
 Epichloe festucae