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Literature summary extracted from

  • Si, M.; Zhao, C.; Zhang, B.; Wei, D.; Chen, K.; Yang, X.; Xiao, H.; Shen, X.
    Overexpression of mycothiol disulfide reductase enhances Corynebacterium glutamicum robustness by modulating cellular redox homeostasis and antioxidant proteins under oxidative stress (2016), Sci. Rep., 6, 29491 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.15 overexpression of His6-tagged enzyme from expression vector pXMJ19 in Corynebacterium glutamicum. Transfected cells show increased tolerance to reactive oxygen species induced by oxidants, bactericidal antibiotics, alkylating agents, and heavy metals Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.15 additional information overexpression of mycothiol disulfide reductase enhances Corynebacterium glutamicum robustness by modulating cellular redox homeostasis and antioxidant proteins under oxidative stress. Overexpression of Mtr causes a marked increase in the ratio of reduced to oxidized mycothiol (MSH:MSSM), and significantly enhances the activities of a variety of antioxidant enzymes, including mycothiol peroxidase (MPx), mycoredoxin 1 (Mrx1), thioredoxin 1 (Trx1), and methionine sulfoxide reductase A (MsrA). The mycothione levels of enzyme expressing cells are lower than those of untransfectde cells under H2O2, ciprofloxacin, CdCl2, N-ethylmaleimide, iodoacetamide, methylglyoxal, and 1-chloro-2,4-dinitrobenzene stresses, no significant difference in MSH or MSSM levels when both cells are exposed to formaldehyde and rifamycin SV Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.15 mycothione + NADPH + H+ Corynebacterium glutamicum
-
 2 mycothiol + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.15 Corynebacterium glutamicum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.15 mycothione + NADPH + H+
-
 Corynebacterium glutamicum 2 mycothiol + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.15 mycothiol disulfide reductase
-
 Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.15 NADPH dependent on Corynebacterium glutamicum

Expression

EC Number Organism Comment Expression
1.8.1.15 Corynebacterium glutamicum enzyme expression is positively regulated by SigH, the stress-responsive extracytoplasmic function-sigma (ECF-sigma) factor up

General Information

EC Number General Information Comment Organism
1.8.1.15 metabolism cellular mycothiol metabolism, overview. Under stress conditions, proteins are oxidized to mixed disulfides with MSH to form S-mycothiolated proteins that is reversed by the Mrx1/Mtr/MSH pathway Corynebacterium glutamicum
1.8.1.15 physiological function the enzyme maintains intracellular redox homeostasis. The physiological roles of Mtr in resistance to oxidative stresses are corroborated by decreased reactive oxygen species levels, reduced carbonylation damage, decreased loss of reduced protein thiols, and a massive increase in the levels of reversible protein thiols in Mtr-overexpressing cells exposed to stressful conditions Corynebacterium glutamicum