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Literature summary extracted from

  • Park, A.; Kim, I.; Do, H.; Jeon, B.; Lee, C.; Roh, S.; Shin, S.; Park, H.; Kim, Y.; Kim, Y.; Yoon, H.; Lee, J.; Kim, H.
    Structure and catalytic mechanism of monodehydroascorbate reductase, MDHAR, from Oryza sativa L. japonica (2016), Sci. Rep., 6, 33903 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.4
-
 Oryza sativa Japonica Group

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.6.5.4 structures in the presence of cofactors, NAD+ and NADP+, and complexed with ascorbic acid and isoascorbic acid. The overall structure of MDHAR is similar to other iron-sulfur protein reductases, except for a unique long loop of 63-80 residues, which seems to be essential in forming the active site pocket. Residue Arg320 plays a major substrate binding role, and Tyr349 mediates electron transfer from NAD(P)H to bound substrate via FAD. The enzymatic activity favours NADH as an electron donor over NADPH Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
1.6.5.4 C70A about 105% of wild-type activity Oryza sativa Japonica Group
1.6.5.4 E196A residue E196 forms a hydrogen bond to the O2B molecule in the adenosine ring of NAD. Mutation reduces catalytic activity. Mutant E196A has only about 3 times higher affinity for NAD than that for NADP, i.e. 16fold increase in affinity for NADP compared to the wild-type Oryza sativa Japonica Group
1.6.5.4 G72N about 70% of wild-type activity Oryza sativa Japonica Group
1.6.5.4 R320A about 20% of wild-type activity Oryza sativa Japonica Group
1.6.5.4 Y349A about 10% of wild-type activity Oryza sativa Japonica Group
1.6.5.4 Y349F about 10% of wild-type activity Oryza sativa Japonica Group
1.6.5.4 Y349W about 10% of wild-type activity Oryza sativa Japonica Group

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.4 Oryza sativa Japonica Group Q652L6
-
-

Synonyms

EC Number Synonyms Comment Organism
1.6.5.4 Os09g0567300
-
 Oryza sativa Japonica Group

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.4 NADH
-
 Oryza sativa Japonica Group
1.6.5.4 NADPH about 50% of the activity with NADH Oryza sativa Japonica Group