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Literature summary extracted from

  • Farzannia, A.; Roghanian, R.; Zarkesh-Esfahani, S.H.; Nazari, M.; Emamzadeh, R.
    FcUni-RLuc an engineered Renilla luciferase with Fc binding ability and light emission activity (2015), Analyst, 140, 1438-1441 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.13.12.5 analysis an advanced Fc-binding probe, FcUni-RLuc, is produced and functionally assayed for labelling IgGs. The Fc antibody binding sequence HWRGWV is fused to Renilla luciferase, and the purified probe is employed for bioluminescence enzyme-linked immunoabsorbance assay of Her2 positive cells Renilla reniformis
1.13.12.5 biotechnology an advanced Fc-binding probe, FcUni-RLuc, is produced and functionally assayed for labelling IgGs. The Fc antibody binding sequence HWRGWV is fused to Renilla luciferase, and the purified probe is employed for bioluminescence enzyme-linked immunoabsorbance assay of Her2 positive cells Renilla reniformis
1.13.12.5 molecular biology an advanced Fc-binding probe, FcUni-RLuc, is produced and functionally assayed for labelling IgGs. The Fc antibody binding sequence HWRGWV is fused to Renilla luciferase, and the purified probe is employed for bioluminescence enzyme-linked immunoabsorbance assay of Her2 positive cells Renilla reniformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.5 recombinant expression of soluble His6-tagged enzyme FcUni-RLuc in Escherichia coli strain BL21 (DE3), an Fc-binding peptide is separated by a five-amino acid linker from RLuc Renilla reniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.5 additional information
-
 additional information Michaelis-Menten kinetics Renilla reniformis
1.13.12.5 0.0025
-
 coelenterazine wild-type enzyme, pH 7.2, 25°C Renilla reniformis
1.13.12.5 0.003
-
 coelenterazine modified enzyme FcUni-RLuc, pH 7.2, 25°C Renilla reniformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.5 coelenterazine + O2 Renilla reniformis
-
 coelenteramide + CO2 + hv
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.5 Renilla reniformis P27652
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.5 recombinant soluble His6-tagged enzyme FcUni-RLuc from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Renilla reniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.5 coelenterazine + O2
-
 Renilla reniformis coelenteramide + CO2 + hv
-
 ?
1.13.12.5 additional information dynamic light scattering has been used for the detection of protein-protein interaction between the IgG antibody and modified enzyme FcUni-RLuc, to which an Fc-binding peptide is bound and separated by a five-amino acid linker from RLuc. Analysis of FcUni-RLuc and Herceptin interaction Renilla reniformis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.12.5 ? x * 36000, recombinant His-tagged enzyme FcUni-RLuc, SDS-PAGE Renilla reniformis

Synonyms

EC Number Synonyms Comment Organism
1.13.12.5 Renilla luciferase
-
 Renilla reniformis
1.13.12.5 RLuc
-
 Renilla reniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.5 25
-
 assay at Renilla reniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.5 7.2
-
 assay at Renilla reniformis