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Literature summary extracted from

  • Xu, L.; Ikeda, H.; Liu, L.; Arakawa, T.; Wakagi, T.; Shoun, H.; Fushinobu, S.
    Structural basis for the 4'-hydroxylation of diclofenac by a microbial cytochrome P450 monooxygenase (2015), Appl. Microbiol. Biotechnol., 99, 3081-3091 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.11 expression in Escherichia coli Streptomyces avermitilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.15.11 in complex with diclofenac, at 2.2 A resolution. The distal pocket of CYP105D7 contains two diclofenac molecules. The C3' and C4' atoms of the dichlorophenyl ring of one diclofenac molecule are positioned near the heme iron Streptomyces avermitilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.11 additional information
-
 diclofenac CYP105D7 binds diclofenac in a slightly cooperative manner with an affinity of 65 microM and a Hill coefficient of 1.16, pH not specified in the publication, temperature not specified in the publication Streptomyces avermitilis

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.11 Streptomyces avermitilis Q825I8
-
-
1.14.15.11 Streptomyces avermitilis DSM 46492 Q825I8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.11 1-deoxypentalenic acid + reduced ferredoxin + O2
-
 Streptomyces avermitilis pentalenic acid + oxidized ferredoxin + H2O
-
 ?
1.14.15.11 1-deoxypentalenic acid + reduced ferredoxin + O2
-
 Streptomyces avermitilis DSM 46492 pentalenic acid + oxidized ferredoxin + H2O
-
 ?
1.14.15.11 diclofenac + reduced ferredoxin + O2
-
 Streptomyces avermitilis 4'-hydroxydiclofenac + oxidized ferredoxin + H2O
-
 ?
1.14.15.11 diclofenac + reduced ferredoxin + O2
-
 Streptomyces avermitilis DSM 46492 4'-hydroxydiclofenac + oxidized ferredoxin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.11 ? x * 45000, SDS-PAGE, recombinant protein with His-tag Streptomyces avermitilis

Synonyms

EC Number Synonyms Comment Organism
1.14.15.11 CYP105D7
-
 Streptomyces avermitilis
1.14.15.11 pentalenic acid synthase
-
 Streptomyces avermitilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.11 heme
-
 Streptomyces avermitilis