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Literature summary extracted from

  • Dilworth, M.; Eldridge, M.; Eady, R.
    The molybdenum and vanadium nltrogenases of Azotobacter chroococcum Effect of elevated temperature on N2 reduction (1993), Biochem. J., 289, 395-400 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.6.2 additional information
-
 additional information over the temperature range 30-45 degrees C the apparent Km for the reduction of N2 is the same, but increases from 30 to 58 kPa of N2 Azotobacter chroococcum

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.2 Azotobacter chroococcum P15332 and P15334 and P15333 P15332 i.e. subunit VnfD, P15334 i.e. subunit VnfK, P15333 i.e. subunit VnfG
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1.18.6.2 Azotobacter chroococcum MCD1 P15332 and P15334 and P15333 P15332 i.e. subunit VnfD, P15334 i.e. subunit VnfK, P15333 i.e. subunit VnfG
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.2 12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O
-
 Azotobacter chroococcum 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate
-
 ?
1.18.6.2 12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O
-
 Azotobacter chroococcum MCD1 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.18.6.2 vnfD alpha-chain Azotobacter chroococcum
1.18.6.2 vnfG delta-chain Azotobacter chroococcum
1.18.6.2 vnfK beta-chain Azotobacter chroococcum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.18.6.2 45 50 on increasing the temperature from 45 degrees C to 50 degrees C, little change occurrs in the rate of reduction of protons to H2. The rate of N2H4 production increases, but the rate of NH3 formation decreases 7fold. This selective loss of the ability to reduce N2 to NH3 is reversible Azotobacter chroococcum