EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.83 | dithiothreitol | 3fold increase in activity in presence of dithiothreitol | Catharanthus roseus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.83 | 4-chloromercuribenzoate | 0.1 mM, 17% residual activity | Catharanthus roseus | |
1.14.14.83 | CO | inhibition increases with increasing CO concentrations and is almost completely reversed with the aid of a light spurce between 420 and 450 nm | Catharanthus roseus | |
1.14.14.83 | iodoacetamide | 10 mM, 87% residual activity | Catharanthus roseus | |
1.14.14.83 | Metyrapone | 0.1 mM, 41% residual activity | Catharanthus roseus | |
1.14.14.83 | N-ethylmaleimide | 1 mM, 58% residual activity | Catharanthus roseus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.83 | 0.0055 | - |
geraniol | pH 7.2, 25°C | Catharanthus roseus | |
1.14.14.83 | 0.011 | - |
nerol | pH 7.2, 25°C | Catharanthus roseus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.83 | membrane | - |
Catharanthus roseus | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.83 | geraniol + [reduced NADPH-hemoprotein reductase] + O2 | Catharanthus roseus | - |
(6E)-8-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.83 | nerol + [reduced NADPH-hemoprotein reductase] + O2 | Catharanthus roseus | - |
(6E)-8-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.83 | Catharanthus roseus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.83 | solubilization by treatment with sodium cholate, Renex-30, or Lubrol-WX | Catharanthus roseus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.83 | seedling | etiolated seedling | Catharanthus roseus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.83 | geraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Catharanthus roseus | (6E)-8-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.83 | nerol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Catharanthus roseus | (6E)-8-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.83 | cytochrome P450 | - |
Catharanthus roseus |