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Literature summary extracted from

  • Segato, F.; Dias, B.; Berto, G.L.; de Oliveira, D.M.; De Souza, F.H.M.; Citadini, A.P.; Murakami, M.T.; Damasio, A.R.L.; Squina, F.M.; Polikarpov, I.
    Cloning, heterologous expression and biochemical characterization of a non-specific endoglucanase family 12 from Aspergillus terreus NIH2624 (2017), Biochim. Biophys. Acta, 1865, 395-403 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 Cs+ 50 mM, 110% of initial activtiy Aspergillus terreus
3.2.1.4 DMSO 0.5%, 108% of initial activtiy Aspergillus terreus
3.2.1.4 K+ 50 mM, 112% of initial activtiy Aspergillus terreus
3.2.1.4 Triton X-100 0.5%, 105% of initial activtiy Aspergillus terreus
3.2.1.4 Tween 0.5%, 1121% of initial activtiy Aspergillus terreus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Aspergillus nidulans Aspergillus terreus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Ca2+ 10 mM, 90% residual activity Aspergillus terreus
3.2.1.4 Cd2+ 10 mM, 16.5% residual activity Aspergillus terreus
3.2.1.4 Cu2+ 10 mM, 27% residual activity Aspergillus terreus
3.2.1.4 EDTA 10 mM, 58% residual activity Aspergillus terreus
3.2.1.4 Fe2+ 10 mM, 30% residual activity Aspergillus terreus
3.2.1.4 Mn2+ 10 mM, 57% residual activity Aspergillus terreus
3.2.1.4 Zn2+ 10 mM, 95% residual activity Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 xyloglucan Km value 12.8 mg/ml, pH 6.5, 50°C Aspergillus terreus
3.2.1.4 additional information
-
 Barley beta-glucan Km value 2.2 mg/ml, pH 6.5, 50°C Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Aspergillus terreus Q0CFW4
-
-
3.2.1.4 Aspergillus terreus NIH 2624 Q0CFW4
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 3.3
-
 substrate carboxymethylcellulose, pH 6.5, 50°C Aspergillus terreus
3.2.1.4 21.8
-
 substrate konjac mannan, pH 6.5, 50°C Aspergillus terreus
3.2.1.4 115
-
 substrate xyloglucan, pH 6.5, 50°C Aspergillus terreus
3.2.1.4 256
-
 substrate barley beta-glucan, pH 6.5, 50°C Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 barley beta-glucan + H2O
-
 Aspergillus terreus ?
-
 ?
3.2.1.4 barley beta-glucan + H2O
-
 Aspergillus terreus NIH 2624 ?
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Aspergillus terreus ?
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Aspergillus terreus NIH 2624 ?
-
 ?
3.2.1.4 konjac mannan + H2O
-
 Aspergillus terreus ?
-
 ?
3.2.1.4 konjac mannan + H2O
-
 Aspergillus terreus NIH 2624 ?
-
 ?
3.2.1.4 xyloglucan + H2O isolated from tamarind Aspergillus terreus ?
-
 ?
3.2.1.4 xyloglucan + H2O isolated from tamarind Aspergillus terreus NIH 2624 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 ATEG_07420
-
 Aspergillus terreus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 55
-
-
 Aspergillus terreus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 50 60 melting temperature Aspergillus terreus
3.2.1.4 60
-
 stable up to 60 min Aspergillus terreus
3.2.1.4 70
-
 20 min, loss of activity Aspergillus terreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5
-
-
 Aspergillus terreus