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Literature summary extracted from

  • Zhang, Y.; Yang, H.; Xin, Y.; Zhang, L.; Wang, W.
    Enhancing xanthine oxidase fermentation with pH-shift strategy based on kinetic analysis by Arthrobacter M3 (2014), Bioprocess Biosyst. Eng., 37, 1899-1905 .
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.3.2 hypoxanthine + NAD+ + H2O Arthrobacter sp.
-
 xanthine + NADH + H+
-
 ?
1.17.3.2 hypoxanthine + NAD+ + H2O Arthrobacter sp. M3
-
 xanthine + NADH + H+
-
 ?
1.17.3.2 xanthine + H2O + O2 Arthrobacter sp.
-
 urate + H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2 Arthrobacter sp. M3
-
 urate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.3.2 Arthrobacter sp.
-
-
-
1.17.3.2 Arthrobacter sp. M3
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.3.2 cell culture optimization of culture condition for enzyme production, e.g. of the pH-shift strategy based on fermentation kinetic analysis. Kinetic models for cell growth, product accumulation, and substrate consumption are also analyzed and used to predict the behavior of fermentation system and to optimize XOD fermentation, optimum initial pH for XOD accumulation is pH 8.6, 30°C, overview Arthrobacter sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.3.2 4.7
-
 cells grown at optimized conditions, pH 8.6, 37°C Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.3.2 hypoxanthine + NAD+ + H2O
-
 Arthrobacter sp. xanthine + NADH + H+
-
 ?
1.17.3.2 hypoxanthine + NAD+ + H2O
-
 Arthrobacter sp. M3 xanthine + NADH + H+
-
 ?
1.17.3.2 xanthine + H2O + O2
-
 Arthrobacter sp. urate + H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2
-
 Arthrobacter sp. M3 urate + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.3.2 XOD
-
 Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.3.2 37
-
 assay at Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.3.2 8.6
-
 assay at Arthrobacter sp.