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Literature summary extracted from
- Production of 10S-hydroxy-8(E)-octadecenoic acid from oleic acid by whole recombinant Escherichia coli cells expressing 10S-dioxygenase from Nostoc punctiforme PCC 73102 with the aid of a chaperone (2017), Biotechnol. Lett., 39, 133-139 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.77 | chaperone Gro7 | activates the enzyme about 200% | Nostoc punctiforme |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.11.77 | recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), coexpression with chaperone Gro7 | Nostoc punctiforme |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.13.11.77 | additional information | optimization of conditions for 10S-hydroxy-8(E)-octadecenoic acid production from oleic acid by recombinant Escherichia coli cells expressing Nostoc punctiforme 10S-dioxygenase with the aid of a chaperone plasmid. Optimal conditions are pH 9.0, 35°C, 15 % v/v dimethyl sulfoxide, 40 g cells/l, and 10 g oleic acid/l. The activity of recombinant cells expressing 10S-dioxygenase is increased by 200% with the aid of chaperone Gro7. Production of 10S-hydroxy-8(E)-octadecenoic acid, 10S-hydroxy-8,12(E,Z)-octadecadienoic acid, and 10S-hydroxy-8,12,15(E,Z,Z)-octadecatrienoic acid from unsaturated fatty acids including oleic acid, linoleic acid, and a-linolenic acid, respectively. The soluble 10S-dioxygenase content in the crude extract of recombinant cells co-expressing the 10S-dioxygenase gene with pGro7 is higher than that of recombinant cells co-expressing pKJE7 or pG-KJE8. Production of 360 mg/g/h 10S-hydroxy-8(E)-octadecenoic acid from oleic acid | Nostoc punctiforme |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.77 | 0.096 | - |
alpha-linolenate | recombinant enzyme, pH 9.0, 35°C | Nostoc punctiforme |  |
| 1.13.11.77 | 0.236 | - |
linoleate | recombinant enzyme, pH 9.0, 35°C | Nostoc punctiforme | |
| 1.13.11.77 | 0.345 | - |
oleate | recombinant enzyme, pH 9.0, 35°C | Nostoc punctiforme | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.77 | alpha-linolenate + O2 | Nostoc punctiforme | - |
(8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate | - |
? | |
| 1.13.11.77 | linoleate + O2 | Nostoc punctiforme | - |
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate | - |
? | |
| 1.13.11.77 | oleate + O2 | Nostoc punctiforme | preferred substrate | (8E,10S)-10-hydroperoxyoctadeca-8-enoate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.77 | Nostoc punctiforme | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.77 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Nostoc punctiforme |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.77 | 0.096 | - |
purified recombinant enzyme, substrate alpha-linolenate, pH 9.0, 35°C | Nostoc punctiforme |
| 1.13.11.77 | 0.236 | - |
purified recombinant enzyme, substrate linoleate, pH 9.0, 35°C | Nostoc punctiforme |
| 1.13.11.77 | 0.345 | - |
purified recombinant enzyme, substrate oleate, pH 9.0, 35°C | Nostoc punctiforme |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.77 | alpha-linolenate + O2 | - |
Nostoc punctiforme | (8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate | - |
? | |
| 1.13.11.77 | linoleate + O2 | - |
Nostoc punctiforme | (8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate | - |
? | |
| 1.13.11.77 | additional information | optimization of assay conditions, overview | Nostoc punctiforme | ? | - |
? | |
| 1.13.11.77 | oleate + O2 | preferred substrate | Nostoc punctiforme | (8E,10S)-10-hydroperoxyoctadeca-8-enoate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.11.77 | ? | x * 63000, recombinant His-tagged enzyme, SDS-PAGE | Nostoc punctiforme |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.77 | 35 | - |
recombinant His-tagged enzyme | Nostoc punctiforme |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.77 | 9 | - |
recombinant His-tagged enzyme | Nostoc punctiforme |
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