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Literature summary extracted from

  • Jensen, C.N.; Mielke, T.; Farrugia, J.E.; Frank, A.; Man, H.; Hart, S.; Turkenburg, J.P.; Grogan, G.
    Structures of the Apo and FAD-bound forms of 2-hydroxybiphenyl 3-monooxygenase (HbpA) locate activity hotspots identified by using directed evolution (2015), ChemBioChem, 16, 968-976 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.44 expression in Escherichia coli BL21 Pseudomonas nitroreducens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.44 single crystals of the enzyme are used to determine its structure in two forms: an FAD-bound form that allows characterisation of the active site, and an apo form that, although lacking flavin, gives extra information on the location of residues and structure of mobile loops that are absent from the FAD complex Pseudomonas nitroreducens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.44 0.0031
-
 2-Hydroxybiphenyl pH 7.5, temperature not specified in the publication, mutant enzyme H48A Pseudomonas nitroreducens
1.14.13.44 0.0033
-
 2-Hydroxybiphenyl pH 7.5, temperature not specified in the publication, wild-type enzyme Pseudomonas nitroreducens
1.14.13.44 0.0048
-
 2-Hydroxybiphenyl pH 7.5, temperature not specified in the publication R242A Pseudomonas nitroreducens
1.14.13.44 0.005
-
 2-Hydroxybiphenyl pH 7.5, temperature not specified in the publication, mutant enzyme D117A Pseudomonas nitroreducens

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.44 Pseudomonas nitroreducens O06647
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.44
-
 Pseudomonas nitroreducens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.44 2-hydroxybiphenyl + NADH + H+ + O2
-
 Pseudomonas nitroreducens 2,3-dihydroxybiphenyl + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.44 tetramer the structure of the tetramer is determined, there is a large cavity at the centre of the tetramer into which the side chains of several hydrophilic amino acids of each monomer, including Glu75 and Tyr76, are projected Pseudomonas nitroreducens

Synonyms

EC Number Synonyms Comment Organism
1.14.13.44 HbpA
-
 Pseudomonas nitroreducens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.44 7.5
-
 assay at Pseudomonas nitroreducens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.44 FAD FAD-dependent monooxygenase Pseudomonas nitroreducens