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Literature summary extracted from

  • Setzke, E.; Hedderich, R.; Heiden, S.; Thauer, R.
    H2 heterodisulfide oxidoreductase complex from Methanobacterium thermoautotrophicum Composition and properties (1994), Eur. J. Biochem., 220, 139-148 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.98.5 coenzyme F430
-
 Methanobacterium thermoautotrophicus
1.8.98.5 potassium phosphate
-
 Methanobacterium thermoautotrophicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.98.5 cytoplasmic membrane
-
 Methanobacterium thermoautotrophicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.98.5 Fe2+ the enzyme contains approximately 26 mol non-heme iron Methanobacterium thermoautotrophicus
1.8.98.5 Ni2+ the enzyme contains approximately 0.6 mol nickel Methanobacterium thermoautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.98.5 250000
-
 gel filtration Methanobacterium thermoautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.98.5 H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB Methanobacterium thermoautotrophicus
-
 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
-
 ?
1.8.98.5 H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB Methanobacterium thermoautotrophicus DSM 2133
-
 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.98.5 Methanobacterium thermoautotrophicus
-
-
-
1.8.98.5 Methanobacterium thermoautotrophicus DSM 2133
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.98.5 DEAE-Sepharose column chromatography, phenyl-Sepharose column chromatography, and hydroxyapatite column chromatography Methanobacterium thermoautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.98.5 H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
 Methanobacterium thermoautotrophicus reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
-
 ?
1.8.98.5 H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
 Methanobacterium thermoautotrophicus DSM 2133 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
-
 ?
1.8.98.5 H2 + oxidized methyl viologen + CoM-S-S-CoB
-
 Methanobacterium thermoautotrophicus reduced methyl viologen + CoB + CoM + H+
-
 ?
1.8.98.5 H2 + oxidized methyl viologen + CoM-S-S-CoB
-
 Methanobacterium thermoautotrophicus DSM 2133 reduced methyl viologen + CoB + CoM + H+
-
 ?
1.8.98.5 additional information no activity with coenzyme F420 Methanobacterium thermoautotrophicus ?
-
 ?
1.8.98.5 additional information no activity with coenzyme F420 Methanobacterium thermoautotrophicus DSM 2133 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.98.5 heterohexamer 2 * 80000 + 2 * 51000 + 2 * 41000 + 2 * 36000 + 2 * 21000 + 2 * 17000, SDS-PAGE Methanobacterium thermoautotrophicus

Synonyms

EC Number Synonyms Comment Organism
1.8.98.5 H2: heterodisulfide oxidoreductase complex
-
 Methanobacterium thermoautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.98.5 FAD the enzyme contains approximately 0.9 mol nickel Methanobacterium thermoautotrophicus