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Literature summary extracted from

  • Stoisser, T.; Rainer, D.; Leitgeb, S.; Wilson, D.K.; Nidetzky, B.
    The Ala95-to-Gly substitution in Aerococcus viridans l-lactate oxidase revisited - structural consequences at the catalytic site and effect on reactivity with O2 and other electron acceptors (2015), FEBS J., 282, 562-578 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.2 expression in Escherichia coli Aerococcus viridans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.3.2 mutant A95G in complex with pyruvate, to 1.65 A resolution. Mutation leads to increased steric volume available in the active site Aerococcus viridans

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.2 A95G mutant is 3fold more reactive towards 2,6-dichlorophenol-indophenol than O2, whereas wildtype is 14fold more reactive towards O2 than 2,6-dichlorophenol-indophenol. Substituted 1,4-benzoquinones are up to 5fold better electron acceptors for reaction with L-lactate-reduced A95G variant than wild-type Aerococcus viridans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.2 additional information
-
 additional information detailed analysis of reductive and oxidative half-reaction Aerococcus viridans

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.2 Aerococcus viridans Q44467
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.2 (S)-2-hydroxybutanoate + O2
-
 Aerococcus viridans 2-oxobutanoate + H2O2
-
 ?
1.1.3.2 (S)-lactate + O2
-
 Aerococcus viridans pyruvate + H2O2
-
 ?
1.1.3.2 (S)-mandelic acid + O2
-
 Aerococcus viridans phenylpyruvate + H2O2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.2 140
-
 (S)-lactate mutant A95G, pH 6.5, 20°C Aerococcus viridans
1.1.3.2 140
-
 (S)-lactate wild-type, pH 6.5, 20°C Aerococcus viridans