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Literature summary extracted from

  • Yang, M.; Zhu, L.; Li, L.; Li, J.; Xu, L.; Fen, J.; Liu, Y.
    Digital gene expression analysis provides insight into the transcript profile of the genes involved in aporphine alkaloid biosynthesis in lotus (Nelumbo nucifera) (2017), Front. Plant Sci., 8, 80 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.102 gene LOC104585940 encoding isozyme 2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis Nelumbo nucifera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.102 (S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2 Nelumbo nucifera
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 (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.102 Nelumbo nucifera A0A1U7Z269 isozyme 2; 2 isozymes, 2 cultivars
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.102 leaf
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 Nelumbo nucifera
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.102 (S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
 Nelumbo nucifera (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.102 (S)-N-methylcoclaurine-3-hydroxylase
-
 Nelumbo nucifera
1.14.14.102 CYP80B
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 Nelumbo nucifera
1.14.14.102 LOC104585940 locus name Nelumbo nucifera
1.14.14.102 N-methylcoclaurine 3'-hydroxylase
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 Nelumbo nucifera
1.14.14.102 NMCH
-
 Nelumbo nucifera

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.102 cytochrome P-450
-
 Nelumbo nucifera
1.14.14.102 NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Nelumbo nucifera

General Information

EC Number General Information Comment Organism
1.14.14.102 metabolism the enzyme is involved in the common pathway of benzylisoquinoline alkaloid biosynthesis and the aporphine alkaloid bisynthesis pathway in lotus, overview. A series of enzymes, norcoclaurine 6-O-methyltransferase (6OMT), coclaurineN-methyltransferase (CNMT), (S)-N-methylcoclaurine-3-hydroxylase (NMCH), and 3-hydroxy-N-methylcoclaurine 4'-O-methyltransferase(4'OMT), exhibit catalytic activity to yield the central branchpoint intermediate (S)-reticuline Nelumbo nucifera
1.14.14.102 physiological function the expression of isozyme 1 is low in the two cultivars, so it may have little effect on alkaloid contents. The expression of CYP80B, NNU08355, has a positive effect on aporphine alkaloids content in lotus, but exhibits phenotypic lag, which might be because of NMCH which acts early in the benzylisoquinoline alkaloid biosynthetic pathway Nelumbo nucifera