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Literature summary extracted from

  • Franklund, C.; Baron, S.; Hylemon, P.
    Characterization of the baiH gene encoding a bile acid-inducible NADH flavin oxidoreductase from Eubacterium sp. strain VPI 12708 (1993), J. Bacteriol., 175, 3002-3012 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.116
-
 [Clostridium] scindens
1.5.1.36
-
 [Clostridium] scindens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.116 1,10-phenanthroline 2.0 mM, 42% inhibition [Clostridium] scindens
1.3.1.116 Acriflavine 0.2 mg/ml, 87% inhibition, acriflavine is a mixture of 3,6-diamino-10-methylacridinium chloride and 3,6-diaminoacridine; 0.2 mM, 13% residual activity [Clostridium] scindens
1.3.1.116 Cu2+ 1 mM, 1% residual activity [Clostridium] scindens
1.3.1.116 CuCl2 1.0 mM, 99% inhibition [Clostridium] scindens
1.3.1.116 EDTA 5.0 mM, 48% inhibition; 5 mM, 52% residual activity [Clostridium] scindens
1.3.1.116 HgCl2 1 mM, 97% inhibition [Clostridium] scindens
1.3.1.116 iodoacetate 1.0 mM, 9% inhibition [Clostridium] scindens
1.3.1.116 N-bromosuccinimide 0.2 mM, 8% residual activity; 0.2 mM, 92% inhibition [Clostridium] scindens
1.3.1.116 N-ethylmaleimide 1.0 mM, 96% inhibition; 1 mM, 4% residual activity [Clostridium] scindens
1.3.1.116 o-phenanthroline 5 mM, 58% residual activity [Clostridium] scindens
1.3.1.116 p-chloromercuriphenyl sulfonate 0.2 mM, 5% residual activity [Clostridium] scindens
1.3.1.116 p-chloromercuriphenyl sulfonic acid 0.2 mM, 95% inhibition [Clostridium] scindens
1.3.1.116 Zn2+ 1 mM, 27% residual activity [Clostridium] scindens
1.3.1.116 ZnCl2 1.0 mM, 73% inhibition [Clostridium] scindens
1.5.1.36 1,10-phenanthroline 2.0 mM, 42% inhibition [Clostridium] scindens
1.5.1.36 Acriflavine 0.2 mg/ml, 87% inhibition, acriflavine is a mixture of 3,6-diamino-10-methylacridinium chloride and 3,6-diaminoacridine [Clostridium] scindens
1.5.1.36 CuCl2 1.0 mM, 99% inhibition [Clostridium] scindens
1.5.1.36 EDTA 5.0 mM, 48% inhibition [Clostridium] scindens
1.5.1.36 HgCl2 1 mM, 97% inhibition [Clostridium] scindens
1.5.1.36 iodoacetate 1.0 mM, 9% inhibition [Clostridium] scindens
1.5.1.36 N-bromosuccinimide 0.2 mM, 92% inhibition [Clostridium] scindens
1.5.1.36 N-ethylmaleimide 1.0 mM, 96% inhibition [Clostridium] scindens
1.5.1.36 p-chloromercuriphenyl sulfonic acid 0.2 mM, 95% inhibition [Clostridium] scindens
1.5.1.36 ZnCl2 1.0 mM, 73% inhibition [Clostridium] scindens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.116 72000
-
 SDS-PAGE [Clostridium] scindens
1.3.1.116 210000
-
 gel filtration [Clostridium] scindens
1.5.1.36 210000
-
 gel filtration [Clostridium] scindens

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.116 [Clostridium] scindens P32370
-
-
1.3.1.116 [Clostridium] scindens JCM 10418 P32370
-
-
1.3.1.116 [Clostridium] scindens VPI 12708 P32370
-
-
1.5.1.36 [Clostridium] scindens P32370
-
-
1.5.1.36 [Clostridium] scindens VPI 12708 P32370
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.116
-
 [Clostridium] scindens
1.5.1.36
-
 [Clostridium] scindens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.116 48.36
-
 pH 6.8, 20°C [Clostridium] scindens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.116 FAD + NADH + H+
-
 [Clostridium] scindens FADH2 + NAD+
-
 ?
1.3.1.116 FAD + NADH + H+
-
 [Clostridium] scindens JCM 10418 FADH2 + NAD+
-
 ?
1.3.1.116 FAD + NADH + H+
-
 [Clostridium] scindens VPI 12708 FADH2 + NAD+
-
 ?
1.5.1.36 reduced flavin + NAD+
-
 [Clostridium] scindens flavin + NADH + H+
-
 ?
1.5.1.36 reduced flavin + NAD+
-
 [Clostridium] scindens JCM 10418 flavin + NADH + H+
-
 ?
1.5.1.36 reduced flavin + NAD+
-
 [Clostridium] scindens VPI 12708 flavin + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.116 homotrimer 3 * 72000, SDS-PAGE [Clostridium] scindens
1.3.1.116 trimer 3 * 72000, SDS-PAGE, x * 72006, calculated from sequence [Clostridium] scindens
1.5.1.36 homotrimer 3 * 72000, SDS-PAGE [Clostridium] scindens

Synonyms

EC Number Synonyms Comment Organism
1.3.1.116 BaiH
-
 [Clostridium] scindens
1.5.1.36 BaiH
-
 [Clostridium] scindens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.116 20
-
 assay at [Clostridium] scindens
1.5.1.36 20
-
 assay at [Clostridium] scindens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.116 6.8
-
 assay at [Clostridium] scindens
1.5.1.36 6.8
-
 assay at [Clostridium] scindens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.116 FAD
-
 [Clostridium] scindens
1.3.1.116 NADH
-
 [Clostridium] scindens

Expression

EC Number Organism Comment Expression
1.3.1.116 [Clostridium] scindens inducible by cholate up
1.5.1.36 [Clostridium] scindens inducible by cholate up