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Literature summary extracted from

  • Yorita, K.; Aki, K.; Ohkuma-Soyejima, T.; Kokubo, T.; Misaki, H.; Massey, V.
    Conversion of L-lactate oxidase to a long chain alpha-hydroxyacid oxidase by site-directed mutagenesis of alanine 95 to glycine (1996), J. Biol. Chem., 271, 28300-28305 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.2 A95G activity with L-lactate similar to wild-type, increased activity with longer chain L-alpha-hydroxyacids such as alpha-hydroxy-n-butyric acid, alpha-hydroxy-n-valeric acid, and also with L-mandelic acid. Reduction of the enzyme bound flavin by substrates is the rate-limiting step in A95G Aerococcus viridans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.2 0.3
-
 (S)-mandelic acid wild-type, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 0.87
-
 L-lactate wild-type, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 5
-
 glycerate wild-type, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 5.5
-
 2-hydroxypentanoate wild-type, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 6.75
-
 L-lactate mutant A95G, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 10
-
 2-hydroxypentanoate mutant A95G, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 18
-
 2-hydroxybutanoate wild-type, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 20
-
 (S)-mandelic acid mutant A95G, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 27
-
 2-hydroxybutanoate mutant A95G, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 53
-
 glycerate mutant A95G, pH 7.0, 25°C Aerococcus viridans

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.2 Aerococcus viridans Q44467
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.2 (S)-mandelic acid + O2
-
 Aerococcus viridans phenylpyruvate + H2O2
-
 ?
1.1.3.2 2-hydroxybutanoate + O2
-
 Aerococcus viridans 2-oxobutanoate + H2O2
-
 ?
1.1.3.2 2-hydroxypentanoate + O2
-
 Aerococcus viridans 2-oxopentanoate + H2O2
-
 ?
1.1.3.2 glycerate + O2
-
 Aerococcus viridans hydroxypyruvate + H2O2
-
 ?
1.1.3.2 L-lactate + O2
-
 Aerococcus viridans pyruvate + H2O2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.2 0.0033
-
 (S)-mandelic acid wild-type, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 0.87
-
 glycerate wild-type, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 1.25
-
 2-hydroxypentanoate wild-type, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 11
-
 2-hydroxybutanoate wild-type, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 15.8
-
 (S)-mandelic acid mutant A95G, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 31.3
-
 glycerate mutant A95G, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 51.7
-
 2-hydroxypentanoate mutant A95G, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 250
-
 L-lactate mutant A95G, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 283
-
 L-lactate wild-type, pH 7.0, 25°C Aerococcus viridans
1.1.3.2 510
-
 2-hydroxybutanoate mutant A95G, pH 7.0, 25°C Aerococcus viridans