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Literature summary extracted from

  • Cao, H.; Pauff, J.M.; Hille, R.
    X-ray crystal structure of a xanthine oxidase complex with the flavonoid inhibitor quercetin (2014), J. Nat. Prod., 77, 1693-1699 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.17.3.2 drug development the enzyme is a target for rational design of flavonoid-type inhibitors against xanthine oxidase useful for the treatment of hyperuricemia, gout, and inflammatory disease states Bos taurus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.17.3.2 enzme in complex with inhibitor quercetin, X-ray diffraction structure determintion and analysis at 2.0 A resolution Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.3.2 additional information natural flavonoids are attractive leads for rational design of preventive and therapeutic xanthine oxidase inhibitors due to their beneficial antioxidant, anti-inflammatory, and antiproliferative activities in addition to their micromolar inhibitory activities toward xanthine oxidase Bos taurus
1.17.3.2 quercetin structure analysis of quercetin in complex with the enzyme, overview. The inhibitor adopts a single orientation with its benzopyran moiety sandwiched between Phe 914 and Phe 1009 and ring B pointing toward the solvent channel leading to the molybdenum active center. The favorable steric complementarity of the conjugated three-ring structure of quercetin with the active site and specific hydrogen-bonding interactions of exocyclic hydroxy groups with catalytically relevant residues Arg 880 and Glu 802 correlate well with a previously reported structure-activity relationship of flavonoid inhibitors of xanthine oxidase Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.3.2 hypoxanthine + NAD+ + H2O Bos taurus
-
 xanthine + NADH + H+
-
 ?
1.17.3.2 xanthine + H2O + O2 Bos taurus
-
 urate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.3.2 Bos taurus P80457
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.3.2 commercial preparation
-
 Bos taurus
-
1.17.3.2 milk
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.3.2 hypoxanthine + NAD+ + H2O
-
 Bos taurus xanthine + NADH + H+
-
 ?
1.17.3.2 xanthine + H2O + O2
-
 Bos taurus urate + H2O2
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.3.2 NAD+
-
 Bos taurus