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Literature summary extracted from

  • You, S.; Zhu, B.; Wang, F.; Han, H.; Sun, M.; Zhu, H.; Peng, R.; Yao, Q.
    A Vitis vinifera xanthine dehydrogenase gene, VvXDH, enhances salinity tolerance in transgenic Arabidopsis (2017), Plant Biotechnol. Rep., 11, 147-160 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.4 gene VvXDH, sequence comparisons and phylogenetic analysis, synthesis of gene VvXDH through PCR-based two-step DNA synthesis method, recombinant overexpression in Arabidopsis thaliana ecotype Columbia under control of CaMV 35S promoter from plasmid pYM817 using the Agrobacterium tumefaciens strain GV3101 transfection method. The transgenic Arabidopsis thaliana plants show enhanced salt tolerance, phenotype, overview. Explicit function of VvXDH in conferring salt stress by increasing allantoin accumulation and activating abscisic acid signaling pathway, enhancing ROS scavenging in transgenic Arabidopsis thaliana. Expression of VvXDH results in elevated allantoin accumulation Vitis vinifera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 xanthine + NAD+ + H2O Vitis vinifera
-
urate + NADH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Vitis vinifera F6GU50
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 xanthine + NAD+ + H2O
-
Vitis vinifera urate + NADH + H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.17.1.4 VvXDH
-
Vitis vinifera
1.17.1.4 XDH
-
Vitis vinifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.4 8.5
-
assay at Vitis vinifera

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 NAD+
-
Vitis vinifera

General Information

EC Number General Information Comment Organism
1.17.1.4 physiological function xanthine dehydrogenase plays an important role in purine catabolism catalyzing the oxidative hydroxylation of hypoxanthine to xanthine and of xanthine to uric acid, it plays a role in recycling and remobilization of nitrogen, and XDH is implicated in plant stress responses and acclimation. Explicit function of VvXDH in conferring salt stress by increasing allantoin accumulation and activating abscisic acid signaling pathway, enhancing ROS scavenging in transgenic Arabidopsis thaliana. VvXDH application results in increased tolerance to abiotic stresses by elevating allantoin accumulation Vitis vinifera