EC Number | Cloned (Comment) | Organism |
---|---|---|
1.18.1.2 | expressed in Escherichia coli Tuner(DE3) cells | Bacillus subtilis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.18.1.2 | additional information | the depletions of residues Y313 to K332 (whole C-terminal extension region) and S325 to K332 results in significant increases in the catalytic efficiency with NADPH in diaphorase assay with ferricyanide, whereas Km values for ferricyanide are increased. In the cytochrome c reduction assay in the presence of ferredoxin, the S325-K332 depleted mutant displays a significant decrease in the turnover rate. The Y313-K332 depleted mutant demonstrates an increase in the rate of the direct reduction of horse heart cytochrome c in the absence of ferredoxin | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.2 | 0.02 | - |
NADPH | with ferricyanide as cosubstrate, at pH 7.0 and 25°C | Bacillus subtilis | |
1.18.1.2 | 0.29 | - |
ferricyanide | at pH 7.0 and 25°C | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.18.1.2 | 97000 | - |
gel filtration | Bacillus subtilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.2 | reduced ferredoxin + NADP+ + H+ | Bacillus subtilis | - |
oxidized ferredoxin + NADPH | - |
r | |
1.18.1.2 | reduced ferredoxin + NADP+ + H+ | Bacillus subtilis 168 | - |
oxidized ferredoxin + NADPH | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.18.1.2 | Bacillus subtilis | - |
- |
- |
1.18.1.2 | Bacillus subtilis 168 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.18.1.2 | hydroxyapatite column chromatography and Mono Q column chromatography | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.2 | ferricyanide + NADPH | - |
Bacillus subtilis | ferrocyanide + NADP+ + H+ | - |
r | |
1.18.1.2 | ferricyanide + NADPH | - |
Bacillus subtilis 168 | ferrocyanide + NADP+ + H+ | - |
r | |
1.18.1.2 | reduced ferredoxin + NADP+ + H+ | - |
Bacillus subtilis | oxidized ferredoxin + NADPH | - |
r | |
1.18.1.2 | reduced ferredoxin + NADP+ + H+ | - |
Bacillus subtilis 168 | oxidized ferredoxin + NADPH | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.18.1.2 | homodimer | 2 * 40000, SDS-PAGE | Bacillus subtilis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.18.1.2 | ferredoxin-NADP+ oxidoreductase | - |
Bacillus subtilis |
1.18.1.2 | FNR | - |
Bacillus subtilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.2 | 930 | - |
NADPH | with ferricyanide as cosubstrate, at pH 7.0 and 25°C | Bacillus subtilis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.18.1.2 | FAD | - |
Bacillus subtilis | |
1.18.1.2 | NADP+ | - |
Bacillus subtilis |