Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chen, C.; Sun, X.; Duanmu, H.; Yu, Y.; Liu, A.; Xiao, J.; Zhu, Y.
    Ectopic expression of a Glycine soja myo-inositol oxygenase gene (GsMIOX1a) in Arabidopsis enhances tolerance to alkaline stress (2015), PLoS ONE, 10, e0129998 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.99.1 gene AtMIOX1, phylogenetic tree, semi-quantitative reverse transcriptase enzyme expression analysis Arabidopsis thaliana
1.13.99.1 gene GsMIOX1a, genetic structure, phylogenetic tree, ectopic expression under control of the constitutive CaMV35S promoter in Arabidopsis thaliana leads to enhanced tolerance to alkaline stress, while expression of enzyme mutant atmiox1 mutant decreases alkaline stress tolerance in Arabidopsis thaliana ecotype Columbia, quantitative real-time PCR enzyme expression analysis. The expression levels of some alkaline stress-responsive and inducible marker genes, including H+-Ppase, NADP-ME, KIN1 and RD29B, are also up-regulated in GsMIOX1a overexpression lines compared with the wild-type and atmiox1 mutant expressing plants Glycine soja

Protein Variants

EC Number Protein Variants Comment Organism
1.13.99.1 additional information generation of an AtMIOX1 gene T-DNA insertion mutant atmiox1, the AtMIOX1 gene is efficiently silenced by the T-DNA insertion in the atmiox1 mutant. Constitutive overepression of Glycine soja MIOX1 under constitutive CaMV35S promoter control can recover the phenotype leading to alkaline stress tolerance in the atmiox1 mutant plants, overview Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.99.1 myo-inositol + O2 Arabidopsis thaliana
-
 D-glucuronate + H2O
-
 ?
1.13.99.1 myo-inositol + O2 Glycine soja
-
 D-glucuronate + H2O
-
 ?
1.13.99.1 myo-inositol + O2 Glycine soja 07256
-
 D-glucuronate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.99.1 Arabidopsis thaliana Q8L799
-
-
1.13.99.1 Glycine soja A0A0B2PCG9
-
-
1.13.99.1 Glycine soja 07256 A0A0B2PCG9
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.99.1 flower GsMIOX1a expressed predominantly in flowers Glycine soja
-
1.13.99.1 additional information GsMIOX1a expression is tissue-specific Glycine soja
-
1.13.99.1 seedling
-
 Arabidopsis thaliana
-
1.13.99.1 seedling
-
 Glycine soja
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.99.1 myo-inositol + O2
-
 Arabidopsis thaliana D-glucuronate + H2O
-
 ?
1.13.99.1 myo-inositol + O2
-
 Glycine soja D-glucuronate + H2O
-
 ?
1.13.99.1 myo-inositol + O2
-
 Glycine soja 07256 D-glucuronate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.99.1 ? x * 36574, sequence calculation Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.13.99.1 AtMIOX
-
 Arabidopsis thaliana
1.13.99.1 GsMIOX1a
-
 Glycine soja
1.13.99.1 inositol oxygenase 1
-
 Arabidopsis thaliana
1.13.99.1 inositol oxygenase 1
-
 Glycine soja
1.13.99.1 MIOX
-
 Glycine soja
1.13.99.1 Myo-inositol oxygenase
-
 Glycine soja

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.99.1 Arabidopsis thaliana sequence calculation
-
 4.93

Expression

EC Number Organism Comment Expression
1.13.99.1 Glycine soja GsMIOX1a is rapidly induced by alkaline stress. Under alkaline stress, GsMIOX1a expression levels increase and reach a maximum level at 6 h that is approximately 42fold higher than that at 0 h up

General Information

EC Number General Information Comment Organism
1.13.99.1 evolution MIOX proteins are highly conserved and present in nearly all eukaryotes Glycine soja
1.13.99.1 malfunction the T-DNA insertion mutant atmiox1 is sensitive to alkaline stress, the phenotype can be complemented by expression of Glycine soja MIOX1 under constitutive CaMV35S promoter control Arabidopsis thaliana
1.13.99.1 physiological function positive function of enzyme GsMIOX1a in the alkaline response in Glycine soja plants, GsMIOX1a gene positively regulates plant tolerance to alkaline stress. Other soybean varieties, including Glycine soja 07256, Glycine soja 50109, Glycine max Suinong 28 and Glycine max Hefeng 55. Glycine max Suinong 28 and Glycine max Hefeng 55 are Chinese soybean cultivars that exhibit much lower adaptability to stress compared to Glycine soja 07256. Glycine soja 50109 is also a kind of wild soybean but is not reported to be alkaline resistant Glycine soja