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Literature summary extracted from

  • Wang, X.; Huang, R.; Quan, R.
    Mutation in Mg-protoporphyrin IX monomethyl ester cyclase decreases photosynthesis capacity in Rice (2017), PLoS ONE, 12, e0171118 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.81 gene OsCRD1, encoding a putative subunit of enzyme MPEC, is a single-copy gene in rice, sequence comparisons, quantitative real-time RT-PCR enzyme expression analysis Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.81 A96T site-directed mutagenesis, the point mutation in OsCRD1 does not change its location, but leads to deficiency in chlorophyll biosynthesis and chloroplast development and decreases photosynthetic capacity in rice, phenotype Oryza sativa Japonica Group
1.14.13.81 G286A naturally occurring mutation, the mutation in enzyme MPEC causes the phenotype of the rice pale-green leaf mutant m167, phenotype Oryza sativa Japonica Group
1.14.13.81 additional information mutant m167 is isolated from a EMS mutagenized population from the Japonica rice variety Kitaake. To construct the F2 mapping population, the yellow-green leaf rice mutant m167 is crossed with rice varieties Zhefu802 and Dular, respectively, genotyping of F2 population Oryza sativa Japonica Group

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.81 chloroplast
-
 Oryza sativa Japonica Group 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.81 magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 Oryza sativa Japonica Group
-
 3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.81 Oryza sativa Japonica Group Q9SDJ2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.81 leaf
-
 Oryza sativa Japonica Group
-
1.14.13.81 leaf sheath
-
 Oryza sativa Japonica Group
-
1.14.13.81 additional information gene OsCRD1 is mainly expressed in green tissues, including stem, leaf, tassel and sheath, but not in root Oryza sativa Japonica Group
-
1.14.13.81 seedling
-
 Oryza sativa Japonica Group
-
1.14.13.81 stem
-
 Oryza sativa Japonica Group
-
1.14.13.81 tassel
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.81 magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2
-
 Oryza sativa Japonica Group 3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.81 LOC_Os01g17170 locus name Oryza sativa Japonica Group
1.14.13.81 Mg-protoporphyrin IX monomethyl ester cyclase
-
 Oryza sativa Japonica Group
1.14.13.81 MPEC
-
 Oryza sativa Japonica Group
1.14.13.81 OsCRD1
-
 Oryza sativa Japonica Group
1.14.13.81 rice copper response defect 1 i.e. OsCRD1, a putative subunit of enzyme MPEC Oryza sativa Japonica Group

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.81 NADPH
-
 Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
1.14.13.81 malfunction mutation of in enzyme MPEC causes the pale-green leaf mutant phenotype of mutant m167 with yellow-green leaves across the whole lifespan. Chlorophyll content decreases by 43-51% and the granal stacks of chloroplasts becomes thinner in m167. Chlorophyll fluorescence parameters, including Fv/Fm (the maximum quantum efficiency of PSII) and quantum yield of PSII (Y(II)), are lower in m167 than those in wild-type plants, and photosynthesis rate decreases by 40% in leaves of m167 mutant compared with wild-type plants, which leads to yield reduction in m167 Oryza sativa Japonica Group