Literature summary extracted from
Vetting, M.; Bareich, D.; Yu, M.; Blanchard, J.
Crystal structure of RimI from Salmonella typhimurium LT2, the GNAT responsible for Nalpha-acetylation of ribosomal protein S18 (2008), Protein Sci., 17, 1781-1790 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.3.1.266 |
expressed in Escherichia coli Rosetta2 (DE3) pLysS cells |
Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.3.1.266 |
in complex with CoA, acetyl-CoA, and a CoA-S-acetyl-ARYFRR bisubstrate inhibitor, vapor diffusion method, using |
Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.3.1.266 |
CoA-S-acetyl-ARYFRR |
linearly competitive against acetyl-CoA and linearly noncompetitive against S18 protein(1-6) |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.3.1.266 |
acetyl-CoA + an N-terminal L-alanyl-[S18 protein of 30S ribosome] |
Salmonella enterica subsp. enterica serovar Typhimurium |
- |
CoA + an N-terminal N-acetyl-L-alanyl-[S18 protein of 30S ribosome] |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.3.1.266 |
Salmonella enterica subsp. enterica serovar Typhimurium |
Q8ZJW4 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.3.1.266 |
Ni HiTrap column chromatography |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.3.1.266 |
acetyl-CoA + an N-terminal L-alanyl-[S18 protein of 30S ribosome] |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
CoA + an N-terminal N-acetyl-L-alanyl-[S18 protein of 30S ribosome] |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.3.1.266 |
RimI |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Ki Value [mM]
EC Number |
Ki Value [mM] |
Ki Value maximum [mM] |
Inhibitor |
Comment |
Organism |
Structure |
---|
2.3.1.266 |
0.0041 |
- |
CoA-S-acetyl-ARYFRR |
at pH 7.3 and 25°C |
Salmonella enterica subsp. enterica serovar Typhimurium |
|