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Literature summary extracted from

  • Lee, S.; Yun, E.J.; Kim, K.H.; Kim, H.Y.; Choi, I.G.
    3,6-Anhydro-L-galactonate cycloisomerase from Vibrio sp. strain EJY3 crystallization and X-ray crystallographic analysis (2017), Acta Crystallogr. Sect. F, , 511-514 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.25 expressed in Escherichia coli BL21(DE3) cells Vibrio sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.5.1.25 hanging drop vapor diffusion method, using 0.2 M magnesium chloride hexahydrate, 0.1 M bis-Tris pH 6.5, 25% (w/v) polyethylene glycol 3350 Vibrio sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.25 3,6-anhydro-L-galactonate Vibrio sp.
-
 2-dehydro-3-deoxy-L-galactonate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.25 Vibrio sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.25 HisTrap column chromatography and Hitrap Q column chromatography Vibrio sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.25 3,6-anhydro-L-galactonate
-
 Vibrio sp. 2-dehydro-3-deoxy-L-galactonate
-
 ir

Subunits

EC Number Subunits Comment Organism
5.5.1.25 monomer
-
 Vibrio sp.

Synonyms

EC Number Synonyms Comment Organism
5.5.1.25 ACI
-
 Vibrio sp.