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Literature summary extracted from

  • Volodina, E.; Steinbuechel, A.
    (S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (FadB') from fatty acid degradation operon of Ralstonia eutropha H16 (2014), AMB Express, 4, 1-9 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.17 expression in Escherichia coli Cupriavidus necator

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.17 acetyl-CoA 0.1 mM, 70% inhibition Cupriavidus necator
4.2.1.17 CoA 0.1 mM, 30% inhibition Cupriavidus necator
4.2.1.17 propionyl-CoA 0.1 mM, 60% inhibition Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.17 87000
-
 gel fitrlation Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.17 Cupriavidus necator Q0KEG0 enzyme shos enoyl-CoA hydratase activity, and a 3-hydroxyacyl-CoA dehydrogenase activity
-
4.2.1.17 Cupriavidus necator DSM 428 Q0KEG0 enzyme shos enoyl-CoA hydratase activity, and a 3-hydroxyacyl-CoA dehydrogenase activity
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.17 98
-
 substrate crotonoyl-CoA, pH 7.0, 30°C Cupriavidus necator

Storage Stability

EC Number Storage Stability Organism
4.2.1.17 -20°C, 50% (v/v) glycerol, more than one month without loss of activity Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.17 (S)-3-hydroxybutyryl-CoA
-
 Cupriavidus necator crotonoyl-CoA + H2O
-
 r
4.2.1.17 (S)-3-hydroxybutyryl-CoA
-
 Cupriavidus necator DSM 428 crotonoyl-CoA + H2O
-
 r
4.2.1.17 crotonoyl-CoA + H2O
-
 Cupriavidus necator (S)-3-hydroxybutyryl-CoA
-
 r
4.2.1.17 crotonoyl-CoA + H2O
-
 Cupriavidus necator DSM 428 (S)-3-hydroxybutyryl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.17 monomer 1 * 85900, calculated from sequence, 1 * 86000, SDS-PAGE Cupriavidus necator

Synonyms

EC Number Synonyms Comment Organism
4.2.1.17 FadB'
-
 Cupriavidus necator
4.2.1.17 H16_A0461
-
 Cupriavidus necator

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.17 6 7
-
 Cupriavidus necator

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.17 Cupriavidus necator calculated from sequence
-
 7.7