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Literature summary extracted from

  • Pollmann, K.; Beil, S.; Pieper, D.H.
    Transformation of chlorinated benzenes and toluenes by Ralstonia sp. strain PS12 tecA (tetrachlorobenzene dioxygenase) and tecB (chlorobenzene dihydrodiol dehydrogenase) gene products (2001), Appl. Environ. Microbiol., 67, 4057-4063 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.119 the tecB gene is cloned together with the tecA gene into Escherichia coli DH5alpha Cupriavidus sp. PS12
1.14.12.26 the tecB gene is cloned into Escherichia coli DH5a together with the tecA gene into Escherichia coli DH5alpha Cupriavidus sp. PS12

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.119 Cupriavidus sp. PS12 O69264
-
-
1.14.12.26 Cupriavidus sp. PS12
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.26 2,3-dichlorotoluene + NADH + H+ + O2
-
 Cupriavidus sp. PS12 2,3-dichlorobenzyl alcohol + NAD+
-
 ?
1.14.12.26 2,4,5-trichlorotoluene + NADH + H+ + O2
-
 Cupriavidus sp. PS12 2,4,5-trichlorobenzyl alcohol + NAD+
-
 ?
1.14.12.26 2,6-dichlorotoluene + NADH + H+ + O2
-
 Cupriavidus sp. PS12 2,6-dichlorobenzyl alcohol + NAD+
-
 ?
1.14.12.26 3,5-dichlorotoluene + NADH + H+ + O2
-
 Cupriavidus sp. PS12 3,5-dichlorobenzyl alcohol + NAD+
-
 ?
1.14.12.26 chlorobenzene + NADH + H+ + O2
-
 Cupriavidus sp. PS12 (1R,2R)-3-chlorocyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.119 tecB
-
 Cupriavidus sp. PS12
1.14.12.26 TecA
-
 Cupriavidus sp. PS12
1.14.12.26 tetrachlorobenzene dioxygenase
-
 Cupriavidus sp. PS12

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.26 NADH
-
 Cupriavidus sp. PS12