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Literature summary extracted from

  • Katane, M.; Ariyoshi, M.; Tateishi, S.; Koiwai, S.; Takaku, K.; Nagai, K.; Nakayama, K.; Saitoh, Y.; Miyamoto, T.; Sekine, M.; Mita, M.; Hamase, K.; Matoba, S.; Homma, H.
    Structural and enzymatic properties of mammalian D-glutamate cyclase (2018), Arch. Biochem. Biophys., 654, 10-18 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.48
-
 Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.48 ADP significantly decreases activity with 5-oxo-D-proline and activity with D-glutamate Mus musculus
4.2.1.48 ATP significantly decreases activity with 5-oxo-D-proline and activity with D-glutamate Mus musculus
4.2.1.48 EDTA
-
 Mus musculus
4.2.1.48 FAD
-
 Mus musculus
4.2.1.48 FMN
-
 Mus musculus
4.2.1.48 Mn2+ completely abolishes activity with 5-oxo-D-proline, stimulates activity with D-glutamate Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.48 31.9
-
 D-glutamate pH 8.0, 37°C Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.48 mitochondrion highly expressed in the mitochondrial matrix Mus musculus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.48 CaCl2 no significant activity with 5-oxo-D-proline, stimulates activity with D-glutamate Mus musculus
4.2.1.48 CuCl2 no significant activity with 5-oxo-D-proline, stimulates activity with D-glutamate Mus musculus
4.2.1.48 MgCl2 no significant activity with 5-oxo-D-proline, stimulates activity with D-glutamate Mus musculus
4.2.1.48 Mn2+ completely abolishes activity with 5-oxo-D-proline, stimulates activity with D-glutamate Mus musculus
4.2.1.48 ZnCl2 no significant activity with 5-oxo-D-proline, stimulates activity with D-glutamate Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.48 146000
-
 non-denaturing PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.48 D-glutamate Mus musculus the metalloenzyme that degrades D-glutamate in the mitochondrial matrix in mammalian cells 5-oxo-D-proline + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.48 Mus musculus Q8BH86
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.48
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.48 heart highly expressed in the mitochondrial matrix in the heart Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.48 5-oxo-D-proline + H2O
-
 Mus musculus D-glutamate
-
 r
4.2.1.48 D-glutamate
-
 Mus musculus 5-oxo-D-proline + H2O
-
 r
4.2.1.48 D-glutamate the metalloenzyme that degrades D-glutamate in the mitochondrial matrix in mammalian cells Mus musculus 5-oxo-D-proline + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.48 dimer 2 * 65874, calculated from sequence Mus musculus

Synonyms

EC Number Synonyms Comment Organism
4.2.1.48 DGLUCY
-
 Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.48 7.67
-
 5-oxo-D-proline pH 8.0, 37°C Mus musculus
4.2.1.48 35.6
-
 D-glutamate pH 8.0, 37°C Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.48 8
-
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.48 6 8 small but significant increase in activity occurrs between pH 6.0 to 8.0 Mus musculus

General Information

EC Number General Information Comment Organism
4.2.1.48 metabolism disrupts D-glutamate and/or 5-oxo-D-proline levels, contributing to the onset and/or exacerbation of heart failure Mus musculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.48 1121
-
 D-glutamate pH 8.0, 37°C Mus musculus