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Literature summary extracted from

  • Myers, R.S.; Jensen, J.R.; Deras, I.L.; Smith, J.L.; Davisson, V.J.
    Substrate-induced changes in the ammonia channel for imidazole glycerol phosphate synthase (2003), Biochemistry, 42, 7013-7022 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.3.2.10 K258A about 2600fold decrease in kcat/Km for glutamine dependent cyclase reaction, basal and stimulated glutaminase functions are not altered. The stoichiometry of reaction glutamine: 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide turnover is 43:1 compared to wild-type value 1:1 Saccharomyces cerevisiae
4.3.2.10 K258R about 20fold decrease in kcat/Km for glutamine dependent cyclase reaction,basal and stimulated glutaminase functions are not altered. The stoichiometry of reaction glutamine: 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide turnover is 43:1 compared to wild-type value 3:1 Saccharomyces cerevisiae
4.3.2.10 K360A synthase activity is similar to wild type. The stoichiometry of the reaction is 3:1 indicating some degree of uncoupling due to loss of ammonia Saccharomyces cerevisiae
4.3.2.10 K360R synthase activity is similar to wild type. The mutant retains both structural and functional integrity of the enzyme in the case of glutaminase functions Saccharomyces cerevisiae
4.3.2.10 R239A a 1000fold reduction in kcat/Km is observed in the glutamine dependent synthase reaction. The stoichiometry of reaction glutamine: 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide turnover is 43:1 compared to wild-type value 122:1 Saccharomyces cerevisiae
4.3.2.10 R239H a 1000fold reduction in kcat/Km is observed in the glutamine dependent synthase reaction. The stoichiometry of reaction glutamine: 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide turnover is 43:1 compared to wild-type value 154:1 Saccharomyces cerevisiae
4.3.2.10 R239K a 1000fold reduction in kcat/Km is observed in the glutamine dependent synthase reaction. The stoichiometry of reaction glutamine: 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide turnover is 43:1 compared to wild-type value 40:1 Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.2.10 albizziin mixed-type inhibitor versus 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide, competitive versus glutamine Saccharomyces cerevisiae
4.3.2.10 rPRFAR-1 competitive inhibitor versus 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide inhibitor, mixed-type inhibitor versus glutamine Saccharomyces cerevisiae
4.3.2.10 rPRFAR-2 competitive versus 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.10 0.0016
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme R239K Saccharomyces cerevisiae
4.3.2.10 0.0018
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K360A Saccharomyces cerevisiae
4.3.2.10 0.002
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K258R Saccharomyces cerevisiae
4.3.2.10 0.0023
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K360R Saccharomyces cerevisiae
4.3.2.10 0.003
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme R239A Saccharomyces cerevisiae
4.3.2.10 0.005
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, wild-type enzyme Saccharomyces cerevisiae
4.3.2.10 0.0079
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme R239H Saccharomyces cerevisiae
4.3.2.10 0.046
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme K258R Saccharomyces cerevisiae
4.3.2.10 0.053
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme R239A Saccharomyces cerevisiae
4.3.2.10 0.055
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, wild-type enzyme Saccharomyces cerevisiae
4.3.2.10 0.065
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme K360A Saccharomyces cerevisiae
4.3.2.10 0.072
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme K360R Saccharomyces cerevisiae
4.3.2.10 0.075
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme R239H Saccharomyces cerevisiae
4.3.2.10 0.08
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme R239K Saccharomyces cerevisiae
4.3.2.10 0.098
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K258A Saccharomyces cerevisiae
4.3.2.10 0.139
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme K258A Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.10 Saccharomyces cerevisiae P33734
-
-
4.3.2.10 Saccharomyces cerevisiae ATCC 204508 P33734
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine 4900fold upregulation of glutamine hydrolysis in the presence of an acceptor substrate. Transfer of ammonia from the glutaminase site occurs through the (beta/alpha)(8) core of the protein. The conserved K258 residue is key to productive binding and the overall stoichiometry of the reaction. The binding of the ribulosyl phosphate portion of the substrate appears to be transduced through reorientation of K258 resulting in a conformational switch at the base of the (beta/alpha)(8) core that enables the passage of ammonia through the core of the protein Saccharomyces cerevisiae 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
 ?
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine 4900fold upregulation of glutamine hydrolysis in the presence of an acceptor substrate. Transfer of ammonia from the glutaminase site occurs through the (beta/alpha)(8) core of the protein. The conserved K258 residue is key to productive binding and the overall stoichiometry of the reaction. The binding of the ribulosyl phosphate portion of the substrate appears to be transduced through reorientation of K258 resulting in a conformational switch at the base of the (beta/alpha)(8) core that enables the passage of ammonia through the core of the protein Saccharomyces cerevisiae ATCC 204508 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
 ?
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + NH4+
-
 Saccharomyces cerevisiae 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H2O
-
 ?
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + NH4+
-
 Saccharomyces cerevisiae ATCC 204508 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.2.10 IGP synthase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.2.10 0.045
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K258A Saccharomyces cerevisiae
4.3.2.10 0.126
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K258R Saccharomyces cerevisiae
4.3.2.10 0.15
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme R239A Saccharomyces cerevisiae
4.3.2.10 0.21
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme R239H Saccharomyces cerevisiae
4.3.2.10 0.24
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K360A Saccharomyces cerevisiae
4.3.2.10 0.29
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K360R Saccharomyces cerevisiae
4.3.2.10 0.3
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme R239K Saccharomyces cerevisiae
4.3.2.10 0.31
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme K360R Saccharomyces cerevisiae
4.3.2.10 0.7
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme K360A Saccharomyces cerevisiae
4.3.2.10 0.845
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, wild-type enzyme Saccharomyces cerevisiae
4.3.2.10 3.9
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme R239H Saccharomyces cerevisiae
4.3.2.10 4.3
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme R239A Saccharomyces cerevisiae
4.3.2.10 5
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme K258R Saccharomyces cerevisiae
4.3.2.10 5.4
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, wild-type enzyme Saccharomyces cerevisiae
4.3.2.10 5.4
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme K258A Saccharomyces cerevisiae
4.3.2.10 8.7
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme R239K Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.2.10 0.039
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme K258A Saccharomyces cerevisiae
4.3.2.10 0.12
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme K258R Saccharomyces cerevisiae
4.3.2.10 0.46
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K258A Saccharomyces cerevisiae
4.3.2.10 0.5
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme R239H Saccharomyces cerevisiae
4.3.2.10 1.4
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme R239A Saccharomyces cerevisiae
4.3.2.10 2.8
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme R239A Saccharomyces cerevisiae
4.3.2.10 2.9
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme R239H Saccharomyces cerevisiae
4.3.2.10 3.8
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme R239K Saccharomyces cerevisiae
4.3.2.10 4.3
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme K360R Saccharomyces cerevisiae
4.3.2.10 5.5
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme R239K Saccharomyces cerevisiae
4.3.2.10 11
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme K360A Saccharomyces cerevisiae
4.3.2.10 60
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K258R Saccharomyces cerevisiae
4.3.2.10 110
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K360R Saccharomyces cerevisiae
4.3.2.10 130
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K360A Saccharomyces cerevisiae
4.3.2.10 150
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: NH4+, wild-type enzyme Saccharomyces cerevisiae
4.3.2.10 1200
-
 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide 30°C, pH 7.0, cosubstrate: L-glutamine, wild-type enzyme Saccharomyces cerevisiae