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Literature summary extracted from

  • Andrews, F.H.; Rogers, M.P.; Paul, L.N.; McLeish, M.J.
    Perturbation of the monomer-monomer interfaces of the benzoylformate decarboxylase tetramer (2014), Biochemistry, 53, 4358-4367 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.7 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.7 hanging drop vapor diffusion method, using 100 mM Tris-HCl (pH 8.5), 150 mM CaCl2, 0.5% (w/v) 2-methyl-2,4-pentanediol, and 22% (w/v) PEG 400 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.7 phenylglyoxylate Pseudomonas putida
-
 benzaldehyde + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.7 Pseudomonas putida P20906
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.7 Ni-NTA column chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.7 (E)-2-oxo-4(pyridin-3-yl)-3-butenoic acid
-
 Pseudomonas putida 3-(pyridin-3-yl)acrylaldehyde + CO2
-
 ?
4.1.1.7 phenylglyoxylate
-
 Pseudomonas putida benzaldehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.7 homotetramer
-
 Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
4.1.1.7 BFDC
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.7 thiamine diphosphate
-
 Pseudomonas putida