Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Busch, F.; Rajendran, C.; Mayans, O.; Loeffler, P.; Merkl, R.; Sterner, R.
    TrpB2 enzymes are O-phospho-L-serine dependent tryptophan synthases (2014), Biochemistry, 53, 6078-6083 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.20 expression in Escherichia coli Arabidopsis thaliana
4.2.1.20 expression in Escherichia coli Thermotoga maritima
4.2.1.20 expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.20 0.01
-
 O-phospho-L-serine pH 7.5, 30°C Arabidopsis thaliana
4.2.1.20 0.014
-
 O-phospho-L-serine isoform TrpB2a, pH 7.5, 60°C Saccharolobus solfataricus
4.2.1.20 0.015
-
 O-phospho-L-serine isoform TrpB2i, pH 7.5, 60°C Saccharolobus solfataricus
4.2.1.20 0.316
-
 O-phospho-L-serine pH 7.5, 80°C Thermotoga maritima
4.2.1.20 35
-
 L-serine pH 7.5, 30°C Arabidopsis thaliana
4.2.1.20 35
-
 L-serine isoform TrpB2i, pH 7.5, 60°C Saccharolobus solfataricus
4.2.1.20 50.2
-
 L-serine pH 7.5, 80°C Thermotoga maritima
4.2.1.20 151
-
 L-serine isoform TrpB2a, pH 7.5, 60°C Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Arabidopsis thaliana
-
-
-
4.2.1.20 Saccharolobus solfataricus Q97TX6 beta-subunit
-
4.2.1.20 Thermotoga maritima
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 L-serine + indole
-
 Arabidopsis thaliana L-tryptophan + H2O
-
 ?
4.2.1.20 L-serine + indole
-
 Thermotoga maritima L-tryptophan + H2O
-
 ?
4.2.1.20 L-serine + indole
-
 Saccharolobus solfataricus L-tryptophan + H2O
-
 ?
4.2.1.20 additional information TrpB2 subunits of tryptophan synthase are O-phospho-L-serine dependent tryptophan synthases, whereas TrpB1 enzymes catalyze the L-serine dependent synthesis of tryptophan Arabidopsis thaliana ?
-
 ?
4.2.1.20 additional information TrpB2 subunits of tryptophan synthase are O-phospho-L-serine dependent tryptophan synthases, whereas TrpB1 enzymes catalyze the L-serine dependent synthesis of tryptophan Thermotoga maritima ?
-
 ?
4.2.1.20 additional information TrpB2 subunits of tryptophan synthase are O-phospho-L-serine dependent tryptophan synthases, whereas TrpB1 enzymes catalyze the L-serine dependent synthesis of tryptophan Saccharolobus solfataricus ?
-
 ?
4.2.1.20 O-phospho-L-serine + indole
-
 Arabidopsis thaliana L-tryptophan + phosphate
-
 ?
4.2.1.20 O-phospho-L-serine + indole
-
 Thermotoga maritima L-tryptophan + phosphate
-
 ?
4.2.1.20 O-phospho-L-serine + indole
-
 Saccharolobus solfataricus L-tryptophan + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.20 TrpB2a
-
 Saccharolobus solfataricus
4.2.1.20 TrpB2i
-
 Saccharolobus solfataricus
4.2.1.20 TrpB2o
-
 Arabidopsis thaliana
4.2.1.20 TrpB2o
-
 Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.20 0.015
-
 O-phospho-L-serine pH 7.5, 30°C Arabidopsis thaliana
4.2.1.20 0.015
-
 O-phospho-L-serine isoform TrpB2a, pH 7.5, 60°C Saccharolobus solfataricus
4.2.1.20 0.016
-
 L-serine pH 7.5, 30°C Arabidopsis thaliana
4.2.1.20 0.032
-
 L-serine isoform TrpB2a, pH 7.5, 60°C Saccharolobus solfataricus
4.2.1.20 0.2
-
 L-serine isoform TrpB2i, pH 7.5, 60°C Saccharolobus solfataricus
4.2.1.20 0.3
-
 O-phospho-L-serine isoform TrpB2i, pH 7.5, 60°C Saccharolobus solfataricus
4.2.1.20 0.414
-
 O-phospho-L-serine pH 7.5, 80°C Thermotoga maritima
4.2.1.20 0.44
-
 L-serine pH 7.5, 80°C Thermotoga maritima

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.20 0.0002
-
 L-serine isoform TrpB2a, pH 7.5, 60°C Saccharolobus solfataricus
4.2.1.20 0.00045
-
 L-serine pH 7.5, 30°C Arabidopsis thaliana
4.2.1.20 0.005
-
 L-serine isoform TrpB2i, pH 7.5, 60°C Saccharolobus solfataricus
4.2.1.20 0.0087
-
 L-serine pH 7.5, 80°C Thermotoga maritima
4.2.1.20 1.1
-
 O-phospho-L-serine isoform TrpB2a, pH 7.5, 60°C Saccharolobus solfataricus
4.2.1.20 1.3
-
 O-phospho-L-serine pH 7.5, 80°C Thermotoga maritima
4.2.1.20 1.5
-
 O-phospho-L-serine pH 7.5, 30°C Arabidopsis thaliana
4.2.1.20 20
-
 O-phospho-L-serine isoform TrpB2i, pH 7.5, 60°C Saccharolobus solfataricus