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Literature summary extracted from

  • Chen, M.; Chou, W.K.; Al-Lami, N.; Faraldos, J.A.; Allemann, R.K.; Cane, D.E.; Christianson, D.W.
    Probing the role of active site water in the sesquiterpene cyclization reaction catalyzed by aristolochene synthase (2016), Biochemistry, 55, 2864-2874 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.3.9 wild type and mutant enzymes in complex with (2E,6E)-farnesyl diphosphate or 4, sitting drop vapor diffusion method, using 20% PEG 3350 as precipitant Aspergillus terreus

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.9 N299A the mutation has decreasing effects on catalysis but significant effects on sesquiterpene product distributions of 50% germacrene A and 50% aristolochene Aspergillus terreus
4.2.3.9 N299A/S303A the decreasing have modest effects on catalysis but significant effects on sesquiterpene product distributions with germacrene A as main product and (E)-nerolidol and aristolochene as by-products Aspergillus terreus
4.2.3.9 N299L the mutation has decreasing effects on sesquiterpene product distributions with germacrene A and aristolochene as main product and (E)-nerolidol as by-product Aspergillus terreus
4.2.3.9 N299V inactive Aspergillus terreus
4.2.3.9 Q151E the mutation has decreasing effects on catalysis but significant effects on sesquiterpene product distributions with aristolochene and germacrene A as main products, and (E)-nerolidol and (E,E)-farnesol as by-products Aspergillus terreus
4.2.3.9 Q151H the mutation has decreasing effects on catalysis but significant effects on sesquiterpene product distributions with germacrene A and (E,E)-farnesol as main products and (E)-nerolidol and aristolochene as by-products Aspergillus terreus
4.2.3.9 S303A the mutation has increasing effects on catalysis and significant effects on sesquiterpene product distributions with germacrene A and (E)-nerolidol as by-products Aspergillus terreus
4.2.3.9 S303D the mutation has decreasing effects on catalysis but significant effects on sesquiterpene product distributions with germacrene A and (E,E)-farnesol as main products and (E)-nerolidol and aristolochene as by-products Aspergillus terreus
4.2.3.9 S303H the mutation has decreasing effects on catalysis but significant effects on sesquiterpene product distributions with germacrene A as main product and (E,E)-farnesol, (E)-nerolidol and aristolochene as by-products Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.9 0.000042
-
 (2E,6E)-farnesyl diphosphate mutant enzyme Q151H, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 0.000061
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303H, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 0.000099
-
 (2E,6E)-farnesyl diphosphate wild type enzyme, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 0.0001
-
 (2E,6E)-farnesyl diphosphate mutant enzyme Q151E, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 0.00012
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303A, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 0.00036
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303D, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 0.00041
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N299A, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 0.00041
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N299A/S303A, at pH 8.0 and 30°C Aspergillus terreus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.9 Mg2+ required Aspergillus terreus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.9 (2E,6E)-farnesyl diphosphate Aspergillus terreus
-
 aristolochene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.9 Aspergillus terreus Q9UR08
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.9 Ni-NTA column chromatography, HiTrap Q column chromatography, and Superdex 26/60 gel filtration Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.9 (2E,6E)-farnesyl diphosphate
-
 Aspergillus terreus aristolochene + diphosphate
-
 ?
4.2.3.9 additional information no activity with farnesyl S-thiolodiphosphate Aspergillus terreus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.9 ATAS
-
 Aspergillus terreus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.9 0.00041
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303D, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 0.00063
-
 (2E,6E)-farnesyl diphosphate mutant enzyme Q151H, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 0.0013
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303H, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 0.0015
-
 (2E,6E)-farnesyl diphosphate mutant enzyme Q151E, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 0.0058
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N299A/S303A, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 0.012
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N299A, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 0.027
-
 (2E,6E)-farnesyl diphosphate wild type enzyme, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 0.045
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303A, at pH 8.0 and 30°C Aspergillus terreus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.9 1.1
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303D, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 14
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N299A/S303A, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 15
-
 (2E,6E)-farnesyl diphosphate mutant enzyme Q151E, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 15
-
 (2E,6E)-farnesyl diphosphate mutant enzyme Q151H, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 21
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303H, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 29
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N299A, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 270
-
 (2E,6E)-farnesyl diphosphate wild type enzyme, at pH 8.0 and 30°C Aspergillus terreus
4.2.3.9 370
-
 (2E,6E)-farnesyl diphosphate mutant enzyme S303A, at pH 8.0 and 30°C Aspergillus terreus