EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.7 | expressed in Escherichia coli Rosetta2(DE3)pLysS cells | Mycolicibacterium smegmatis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.1.1.7 | sitting drop vapor diffusion method, using 4.0 M sodium formate | Mycolicibacterium smegmatis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.7 | I466A | the mutant shows increased catalytic efficiency for benzoylformate compared to the wild type enzyme | Mycolicibacterium smegmatis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.7 | 2 | 3 | benzoylformate | wild type enzyme, at pH 6.0 and 30°C | Mycolicibacterium smegmatis | |
4.1.1.7 | 15 | - |
benzoylformate | mutant enzyme I466A, at pH 6.0 and 30°C | Mycolicibacterium smegmatis | |
4.1.1.7 | 21 | - |
2-Oxohexanoate | wild type enzyme, at pH 6.0 and 30°C | Mycolicibacterium smegmatis | |
4.1.1.7 | 21 | - |
4-methylthio-2-oxobutanoate | wild type enzyme, at pH 6.0 and 30°C | Mycolicibacterium smegmatis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.7 | Mycolicibacterium smegmatis | - |
- |
- |
4.1.1.7 | Mycolicibacterium smegmatis mc2155 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.7 | HiTrap Ni2+ chelating column chromatography | Mycolicibacterium smegmatis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.7 | 2-oxohexanoate | best substrate | Mycolicibacterium smegmatis | valeraldehyde + CO2 | - |
? | |
4.1.1.7 | 2-oxohexanoate | best substrate | Mycolicibacterium smegmatis mc2155 | valeraldehyde + CO2 | - |
? | |
4.1.1.7 | 4-methylthio-2-oxobutanoate | - |
Mycolicibacterium smegmatis | 3-(methylsulfanyl)propanal + CO2 | - |
? | |
4.1.1.7 | 4-methylthio-2-oxobutanoate | - |
Mycolicibacterium smegmatis mc2155 | 3-(methylsulfanyl)propanal + CO2 | - |
? | |
4.1.1.7 | benzoylformate | - |
Mycolicibacterium smegmatis | benzaldehyde + CO2 | - |
? | |
4.1.1.7 | benzoylformate | - |
Mycolicibacterium smegmatis mc2155 | benzaldehyde + CO2 | - |
? | |
4.1.1.7 | additional information | very low activity with pyruvate, phenylpyruvate, 2-2oxopentanoate, 3-methyl-2-oxopentanoate, 2-oxobutanoate, and 3-methyl-2-oxobutanoate. No activity with 2-oxoglutarate | Mycolicibacterium smegmatis | ? | - |
? | |
4.1.1.7 | additional information | very low activity with pyruvate, phenylpyruvate, 2-2oxopentanoate, 3-methyl-2-oxopentanoate, 2-oxobutanoate, and 3-methyl-2-oxobutanoate. No activity with 2-oxoglutarate | Mycolicibacterium smegmatis mc2155 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.7 | BFDC | - |
Mycolicibacterium smegmatis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.7 | 4.6 | - |
benzoylformate | mutant enzyme I466A, at pH 6.0 and 30°C | Mycolicibacterium smegmatis | |
4.1.1.7 | 5.1 | - |
benzoylformate | wild type enzyme, at pH 6.0 and 30°C | Mycolicibacterium smegmatis | |
4.1.1.7 | 20 | - |
2-Oxohexanoate | wild type enzyme, at pH 6.0 and 30°C | Mycolicibacterium smegmatis | |
4.1.1.7 | 20 | - |
4-methylthio-2-oxobutanoate | wild type enzyme, at pH 6.0 and 30°C | Mycolicibacterium smegmatis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.7 | thiamine diphosphate | - |
Mycolicibacterium smegmatis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.7 | 0.22 | - |
benzoylformate | wild type enzyme, at pH 6.0 and 30°C | Mycolicibacterium smegmatis | |
4.1.1.7 | 0.3 | - |
benzoylformate | mutant enzyme I466A, at pH 6.0 and 30°C | Mycolicibacterium smegmatis | |
4.1.1.7 | 0.94 | - |
2-Oxohexanoate | wild type enzyme, at pH 6.0 and 30°C | Mycolicibacterium smegmatis | |
4.1.1.7 | 0.95 | - |
4-methylthio-2-oxobutanoate | wild type enzyme, at pH 6.0 and 30°C | Mycolicibacterium smegmatis |