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Literature summary extracted from

  • Shukla, H.; Kumar, V.; Singh, A.K.; Singh, N.; Kashif, M.; Siddiqi, M.I.; Yasoda Krishnan, M.; Sohail Akhtar, M.
    Insight into the structural flexibility and function of Mycobacterium tuberculosis isocitrate lyase (2015), Biochimie, 110, 73-80 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.1 expressed in Escherichia coli C41(DE3) cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.1 E423A inactive Mycobacterium tuberculosis
4.1.3.1 E423A/E424A inactive Mycobacterium tuberculosis
4.1.3.1 E423D the mutant shows about 75% of wild type enzyme activity Mycobacterium tuberculosis
4.1.3.1 E423D/E424D the mutant shows about 60% of wild type enzyme activity Mycobacterium tuberculosis
4.1.3.1 E423K inactive Mycobacterium tuberculosis
4.1.3.1 E423K/E424K inactive Mycobacterium tuberculosis
4.1.3.1 E423L inactive Mycobacterium tuberculosis
4.1.3.1 E424A inactive Mycobacterium tuberculosis
4.1.3.1 E424D the mutant shows about 70% of wild type enzyme activity Mycobacterium tuberculosis
4.1.3.1 E424K inactive Mycobacterium tuberculosis
4.1.3.1 E424L inactive Mycobacterium tuberculosis
4.1.3.1 H180A the mutation leads to the destabilization and formation of inactive monomeric enzyme Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 1.142
-
 threo-DL-Isocitrate wild type enzyme, at pH 7.5 and 30°C Mycobacterium tuberculosis
4.1.3.1 1.423
-
 threo-DL-Isocitrate mutant enzyme E423D, at pH 7.5 and 30°C Mycobacterium tuberculosis
4.1.3.1 1.861
-
 threo-DL-Isocitrate mutant enzyme E424D, at pH 7.5 and 30°C Mycobacterium tuberculosis
4.1.3.1 2.031
-
 threo-DL-Isocitrate mutant enzyme E423D/E424D, at pH 7.5 and 30°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 192000
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Mycobacterium tuberculosis
-
 succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate Mycobacterium tuberculosis H37Rv
-
 succinate + glyoxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Mycobacterium tuberculosis P9WKK7
-
-
4.1.3.1 Mycobacterium tuberculosis H37Rv P9WKK7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1 Ni-NTA column chromatography Mycobacterium tuberculosis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate
-
 Mycobacterium tuberculosis succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Mycobacterium tuberculosis H37Rv succinate + glyoxylate
-
 ?
4.1.3.1 threo-DL-isocitrate
-
 Mycobacterium tuberculosis succinate + glyoxylate
-
 ?
4.1.3.1 threo-DL-isocitrate
-
 Mycobacterium tuberculosis H37Rv succinate + glyoxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.1 homotetramer 4 * 48000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
4.1.3.1 ICL
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.1 7.53
-
 threo-DL-Isocitrate mutant enzyme E423D/E424D, at pH 7.5 and 30°C Mycobacterium tuberculosis
4.1.3.1 8.34
-
 threo-DL-Isocitrate mutant enzyme E423D, at pH 7.5 and 30°C Mycobacterium tuberculosis
4.1.3.1 8.46
-
 threo-DL-Isocitrate mutant enzyme E424D, at pH 7.5 and 30°C Mycobacterium tuberculosis
4.1.3.1 9.78
-
 threo-DL-Isocitrate wild type enzyme, at pH 7.5 and 30°C Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
4.1.3.1 physiological function isocitrate lyase is a key enzyme of the glyoxylate shunt crucial for the survival of Mycobacterium tuberculosis in macrophages during persistent infection Mycobacterium tuberculosis