EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.202 | synthesis | Escherichia coli is engineered to produce 1,3-propanediol from glycerol, an inexpensive carbon source. This is done by introducing a synthetic 1,3-propanediol production pathway in recombinant Escherichia coli consisting of glycerol dehydratase complex (dhaB123) and glycerol dehydratase reactivation factors (gdrAB) from Klebsiella pneumoniae and 1,3-propanediol oxidoreductase isoenzyme (yqhD) from Escherichia coli. When 10 mM succinate is added to the medium, the titer of 1,3-propanediol and the glycerol consumption increase to 9.9 and 23.84 g/l, respectively. In addition, the ratio of NADH to NAD+ increases by 43%. Succinate addition is a promising route for the biotechnological production of NADH-dependent microbial metabolites | Klebsiella pneumoniae |
1.1.1.202 | synthesis | NADH-dependent 1,3-propanediol oxidoreductase is a key enzyme for the production of 1,3-propanediol in soluble cell extract. Klebsiella pneumoniae J2B shows a high potential for the production of 1,3-propanediol from glycerol. Optimization of the culture conditions and the elimination of lactate synthesis improves 1,3-propanediol production significantly | Klebsiella pneumoniae |
4.2.1.30 | synthesis | glycerol dehydratase is a key enzyme for the production of 1,3-propanediol in soluble cell extract. Klebsiella pneumoniae J2B shows a high potential for the production of 1,3-propanediol from glycerol. Optimization of the culture conditions and the elimination of lactate synthesis improves 1,3-propanediol production significantly | Klebsiella pneumoniae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.202 | a synthetic pathway 1,3-propanediol production pathway is introduced in recombinant Escherichia coli consisting of glycerol dehydratase complex (dhaB123) and glycerol dehydratase reactivation factors (gdrAB) from Klebsiella pneumoniae and 1,3-propanediol oxidoreductase isoenzyme (yqhD) from Escherichia coli | Klebsiella pneumoniae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.202 | Klebsiella pneumoniae | - |
- |
- |
1.1.1.202 | Klebsiella pneumoniae | A7LGK0 | - |
- |
1.1.1.202 | Klebsiella pneumoniae J2B | - |
- |
- |
4.2.1.30 | Klebsiella pneumoniae | Q59476 AND O08505 AND Q59475 | Q59476: alpha-subunit, O08505: beta-subunit, Q59475: gamma-subunit | - |
4.2.1.30 | Klebsiella pneumoniae J2B | Q59476 AND O08505 AND Q59475 | Q59476: alpha-subunit, O08505: beta-subunit, Q59475: gamma-subunit | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.202 | 3-hydroxypropanal + NADH + H+ | - |
Klebsiella pneumoniae | propane-1,3-diol + NAD+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.202 | DhaT | - |
Klebsiella pneumoniae |
1.1.1.202 | NADH-dependent 1,3-propanediol oxidoreductase | - |
Klebsiella pneumoniae |
1.1.1.202 | YqhD | - |
Klebsiella pneumoniae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.30 | coenzyme B12 | dependent on | Klebsiella pneumoniae |