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Literature summary extracted from

  • Seo, M.J.; Choi, J.H.; Kang, S.H.; Shin, K.C.; Oh, D.K.
    Characterization of L-rhamnose isomerase from Clostridium stercorarium and its application to the production of D-allose from D-allulose (D-psicose) (2018), Biotechnol. Lett., 40, 325-334 .
    View publication on PubMed

Application

EC Number Application Comment Organism
5.3.1.14 synthesis under optimized conditions of pH 7, 70°C, 1 mM Mn2+, 27 U enzyme/l, and 600 g D-allulose/l, 199 g D-allose/l is produced without byproducts over 2.5 h, with a conversion yield of 33% and a productivity of 79.6 g/l/h Thermoclostridium stercorarium subsp. stercorarium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.14 Mn2+ 1 mM, optimum concentration, 7fold increase in activity Thermoclostridium stercorarium subsp. stercorarium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.14 154000
-
 gel filtration Thermoclostridium stercorarium subsp. stercorarium

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.14 Thermoclostridium stercorarium subsp. stercorarium L7VQ35
-
-
5.3.1.14 Thermoclostridium stercorarium subsp. stercorarium DSM 8532 L7VQ35
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.14 D-allose
-
 Thermoclostridium stercorarium subsp. stercorarium D-psicose
-
 r
5.3.1.14 D-allose
-
 Thermoclostridium stercorarium subsp. stercorarium DSM 8532 D-psicose
-
 r
5.3.1.14 D-allulose best ketose substrate Thermoclostridium stercorarium subsp. stercorarium D-psicose
-
 r
5.3.1.14 D-ribose
-
 Thermoclostridium stercorarium subsp. stercorarium D-ribulose
-
 r
5.3.1.14 D-ribose
-
 Thermoclostridium stercorarium subsp. stercorarium DSM 8532 D-ribulose
-
 r
5.3.1.14 D-ribulose
-
 Thermoclostridium stercorarium subsp. stercorarium D-ribose
-
 r
5.3.1.14 L-fructose
-
 Thermoclostridium stercorarium subsp. stercorarium L-mannose
-
 r
5.3.1.14 L-lyxose
-
 Thermoclostridium stercorarium subsp. stercorarium L-xylulose
-
 r
5.3.1.14 L-lyxose
-
 Thermoclostridium stercorarium subsp. stercorarium DSM 8532 L-xylulose
-
 r
5.3.1.14 L-Mannose
-
 Thermoclostridium stercorarium subsp. stercorarium L-Fructose
-
 r
5.3.1.14 L-Mannose
-
 Thermoclostridium stercorarium subsp. stercorarium DSM 8532 L-Fructose
-
 r
5.3.1.14 L-Rhamnose best aldose substrate Thermoclostridium stercorarium subsp. stercorarium L-Rhamnulose
-
 r
5.3.1.14 L-Rhamnose best aldose substrate Thermoclostridium stercorarium subsp. stercorarium DSM 8532 L-Rhamnulose
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.14 trimer 3 * 49000, SDS-PAGE, 3 * 48998, calculated from sequence Thermoclostridium stercorarium subsp. stercorarium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.14 75
-
-
 Thermoclostridium stercorarium subsp. stercorarium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.14 65
-
 half-life 22.8 h Thermoclostridium stercorarium subsp. stercorarium
5.3.1.14 75
-
 half-life 1.9 h Thermoclostridium stercorarium subsp. stercorarium
5.3.1.14 80
-
 half-life 0.2 h Thermoclostridium stercorarium subsp. stercorarium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.14 7
-
-
 Thermoclostridium stercorarium subsp. stercorarium