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Literature summary extracted from
- Cloning and heterologous expression of the cyclooctatin biosynthetic gene cluster affordxa0a diterpene cyclase and two P450 hydroxylases (2009), Chem. Biol., 16, 736-743 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.99.61 | overexpression of the cyclooctatin biosynthetic gene cluster in Escherichia coli | Streptomyces melanosporofaciens |
| 1.14.99.62 | overexpression of the cyclooctatin biosynthetic gene cluster in Escherichia coli | Streptomyces melanosporofaciens |
| 2.5.1.29 | overexpression of the cyclooctatin biosynthetic gene cluster in Escherichia coli | Streptomyces melanosporofaciens |
| 4.2.3.146 | overexpression of the cyclooctatin biosynthetic gene cluster in Escherichia coli | Streptomyces melanosporofaciens |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.146 | 78000 | - |
gel filtration | Streptomyces melanosporofaciens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.61 | cyclooctat-9-en-7-ol + reduced acceptor + O2 | Streptomyces melanosporofaciens | the enzyme is involved in the biosynthesis of cyclooctatin | cyclooctat-9-ene-5,7-diol + acceptor + H2O | - |
? |  |
| 1.14.99.61 | cyclooctat-9-en-7-ol + reduced acceptor + O2 | Streptomyces melanosporofaciens MI614-43F2 | the enzyme is involved in the biosynthesis of cyclooctatin | cyclooctat-9-ene-5,7-diol + acceptor + H2O | - |
? | |
| 1.14.99.62 | cyclooctat-9-ene-5,7-diol + reduced acceptor + O2 | Streptomyces melanosporofaciens | the enzyme is involved in biosynthesis of cyclooctatin | cyclooctatin + acceptor + H2O | - |
? | |
| 1.14.99.62 | cyclooctat-9-ene-5,7-diol + reduced acceptor + O2 | Streptomyces melanosporofaciens M1614-43f2 | the enzyme is involved in biosynthesis of cyclooctatin | cyclooctatin + acceptor + H2O | - |
? | |
| 2.5.1.29 | (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | Streptomyces melanosporofaciens | the enzyme is involved in biosynthesis of cyclooctatin | diphosphate + geranylgeranyl diphosphate | - |
? | |
| 2.5.1.29 | (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | Streptomyces melanosporofaciens M1614-43f2 | the enzyme is involved in biosynthesis of cyclooctatin | diphosphate + geranylgeranyl diphosphate | - |
? | |
| 4.2.3.146 | geranylgeranyl diphosphate + H2O | Streptomyces melanosporofaciens | - |
cyclooctat-9-en-7-ol + diphosphate | - |
? | |
| 4.2.3.146 | geranylgeranyl diphosphate + H2O | Streptomyces melanosporofaciens M1614-43f2 | - |
cyclooctat-9-en-7-ol + diphosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.99.61 | Streptomyces melanosporofaciens | C9K1X6 | - |
- |
| 1.14.99.61 | Streptomyces melanosporofaciens MI614-43F2 | C9K1X6 | - |
- |
| 1.14.99.62 | Streptomyces melanosporofaciens | C9K1X7 | - |
- |
| 1.14.99.62 | Streptomyces melanosporofaciens M1614-43f2 | C9K1X7 | - |
- |
| 2.5.1.29 | Streptomyces melanosporofaciens | C9K1X4 | - |
- |
| 2.5.1.29 | Streptomyces melanosporofaciens M1614-43f2 | C9K1X4 | - |
- |
| 4.2.3.146 | Streptomyces melanosporofaciens | C9K1X5 | - |
- |
| 4.2.3.146 | Streptomyces melanosporofaciens M1614-43f2 | C9K1X5 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.61 | cyclooctat-9-en-7-ol + reduced acceptor + O2 | - |
Streptomyces melanosporofaciens | cyclooctat-9-ene-5,7-diol + acceptor + H2O | - |
? | |
| 1.14.99.61 | cyclooctat-9-en-7-ol + reduced acceptor + O2 | the enzyme is involved in the biosynthesis of cyclooctatin | Streptomyces melanosporofaciens | cyclooctat-9-ene-5,7-diol + acceptor + H2O | - |
? | |
| 1.14.99.61 | cyclooctat-9-en-7-ol + reduced acceptor + O2 | - |
Streptomyces melanosporofaciens MI614-43F2 | cyclooctat-9-ene-5,7-diol + acceptor + H2O | - |
? | |
| 1.14.99.61 | cyclooctat-9-en-7-ol + reduced acceptor + O2 | the enzyme is involved in the biosynthesis of cyclooctatin | Streptomyces melanosporofaciens MI614-43F2 | cyclooctat-9-ene-5,7-diol + acceptor + H2O | - |
? | |
| 1.14.99.62 | cyclooctat-9-ene-5,7-diol + reduced acceptor + O2 | - |
Streptomyces melanosporofaciens | cyclooctatin + acceptor + H2O | - |
? | |
| 1.14.99.62 | cyclooctat-9-ene-5,7-diol + reduced acceptor + O2 | the enzyme is involved in biosynthesis of cyclooctatin | Streptomyces melanosporofaciens | cyclooctatin + acceptor + H2O | - |
? | |
| 1.14.99.62 | cyclooctat-9-ene-5,7-diol + reduced acceptor + O2 | - |
Streptomyces melanosporofaciens M1614-43f2 | cyclooctatin + acceptor + H2O | - |
? | |
| 1.14.99.62 | cyclooctat-9-ene-5,7-diol + reduced acceptor + O2 | the enzyme is involved in biosynthesis of cyclooctatin | Streptomyces melanosporofaciens M1614-43f2 | cyclooctatin + acceptor + H2O | - |
? | |
| 2.5.1.29 | (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Streptomyces melanosporofaciens | diphosphate + geranylgeranyl diphosphate | - |
? | |
| 2.5.1.29 | (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | the enzyme is involved in biosynthesis of cyclooctatin | Streptomyces melanosporofaciens | diphosphate + geranylgeranyl diphosphate | - |
? | |
| 2.5.1.29 | (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Streptomyces melanosporofaciens M1614-43f2 | diphosphate + geranylgeranyl diphosphate | - |
? | |
| 2.5.1.29 | (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | the enzyme is involved in biosynthesis of cyclooctatin | Streptomyces melanosporofaciens M1614-43f2 | diphosphate + geranylgeranyl diphosphate | - |
? | |
| 4.2.3.146 | geranylgeranyl diphosphate + H2O | - |
Streptomyces melanosporofaciens | cyclooctat-9-en-7-ol + diphosphate | - |
? | |
| 4.2.3.146 | geranylgeranyl diphosphate + H2O | the enzyme is involved in biosynthesis of cyclooctatin | Streptomyces melanosporofaciens | cyclooctat-9-en-7-ol + diphosphate | - |
? | |
| 4.2.3.146 | geranylgeranyl diphosphate + H2O | - |
Streptomyces melanosporofaciens M1614-43f2 | cyclooctat-9-en-7-ol + diphosphate | - |
? | |
| 4.2.3.146 | geranylgeranyl diphosphate + H2O | the enzyme is involved in biosynthesis of cyclooctatin | Streptomyces melanosporofaciens M1614-43f2 | cyclooctat-9-en-7-ol + diphosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.3.146 | dimer | 2 * 34000, SDS-PAGE | Streptomyces melanosporofaciens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.99.61 | CotB3 | - |
Streptomyces melanosporofaciens |
| 1.14.99.62 | CotB4 | - |
Streptomyces melanosporofaciens |
| 2.5.1.29 | CotB1 | - |
Streptomyces melanosporofaciens |
| 2.5.1.29 | GGDP synthase | - |
Streptomyces melanosporofaciens |
| 4.2.3.146 | CotB2 | - |
Streptomyces melanosporofaciens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.61 | cytochrome P450 | - |
Streptomyces melanosporofaciens | |
| 1.14.99.62 | cytochrome P450 | - |
Streptomyces melanosporofaciens |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.99.61 | metabolism | the enzyme is involved in the biosynthesis of cyclooctatin | Streptomyces melanosporofaciens |
| 1.14.99.62 | metabolism | the enzyme is involved in biosynthesis of cyclooctatin | Streptomyces melanosporofaciens |
| 2.5.1.29 | metabolism | the enzyme is involved in biosynthesis of cyclooctatin | Streptomyces melanosporofaciens |
| 4.2.3.146 | metabolism | the enzyme is involved in biosynthesis of cyclooctatin | Streptomyces melanosporofaciens |
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