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Literature summary extracted from

  • Asada, Y.; Endo, S.; Inoue, Y.; Mamiya, H.; Hara, A.; Kunishima, N.; Matsunaga, T.
    Biochemical and structural characterization of a short-chain dehydrogenase/reductase of Thermus thermophilus HB8. A hyperthermostable aldose-1-dehydrogenase with broad substrate specificity (2009), Chem. Biol. Interact., 178, 117-126 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.121 overexpression in Escherichia coli Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.121 oil-microbatch method, crystal structure of the enzyme-NAD+ binary complex at 1.65 A resolution. The structure provides evidence for the strict coenzyme specificity and broad substrate specificity of the enzyme Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.121 D242N mutation results in a large decrease in thermostability without dissociation into dimer or monomer Thermus thermophilus
1.1.1.121 F141A mutation results in decrease in thermostability without dissociation into dimer or monomer Thermus thermophilus
1.1.1.121 F249A mutation results in decrease in thermostability without dissociation into dimer or monomer Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.121 1,10-phenanthroline competitive inhibition Thermus thermophilus
1.1.1.121 2,3-benzofuran competitive inhibition Thermus thermophilus
1.1.1.121 Hexestrol competitive inhibition Thermus thermophilus
1.1.1.121 indole competitive inhibition Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.121 0.14
-
 1-Acenaphthenol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.17
-
 (S)-tetralol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.25
-
 4-chromanol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.65
-
 (S)-indanol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 1.3
-
 (R)-indanol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 2 3 D-galactosamine pH 7.4, 50°C Thermus thermophilus
1.1.1.121 2.5
-
 (R)-tetralol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 3.7
-
 2-Cyclohexen-1-ol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 5.5
-
 D-fucose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 27
-
 cis-benzene dihydrodiol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 33
-
 2-deoxy-D-galactose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 78
-
 D-galactose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 131
-
 D-talose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 139
-
 D-glucosamine pH 7.4, 50°C Thermus thermophilus
1.1.1.121 162
-
 L-fucose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 178
-
 6-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 250
-
 cellobiose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 262
-
 L-arabinose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 300
-
 2-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 580
-
 D-glucose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 781
-
 lactose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 841
-
 D-xylose pH 7.4, 50°C Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.121 10000
-
 gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.121 Thermus thermophilus Q5SLC4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.121
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.121 (R)-indanol + NAD+
-
 Thermus thermophilus 2,3-dihydro-1H-inden-1-one + NADH + H+
-
 ?
1.1.1.121 (R)-tetralol + NAD+
-
 Thermus thermophilus 3,4-dihydronaphthalen-2(1H)-one + NADH + H+
-
 ?
1.1.1.121 (S)-indanol + NAD+
-
 Thermus thermophilus 2,3-dihydro-1H-inden-1-one + NADH + H+
-
 ?
1.1.1.121 (S)-tetralol + NAD+
-
 Thermus thermophilus 3,4-dihydronaphthalen-2(1H)-one + NADH + H+
-
 ?
1.1.1.121 1-acenaphthenol + NAD+
-
 Thermus thermophilus ? + NADH + H+
-
 ?
1.1.1.121 2-cyclohexen-1-ol + NAD+
-
 Thermus thermophilus 2-cyclohexen-1-one + NADH + H+
-
 ?
1.1.1.121 2-deoxy-D-galactose + NAD+
-
 Thermus thermophilus 2-deoxy-D-galactono-delta-lactone + NADH + H+
-
 ?
1.1.1.121 2-deoxy-D-glucose + NAD+
-
 Thermus thermophilus 2-deoxy-D-glucono-delta-lactone + NADH + H+
-
 ?
1.1.1.121 4-chromanol + NAD+
-
 Thermus thermophilus 2,3-dihydro-4H-chromen-4-one + NADH + H+
-
 ?
1.1.1.121 6-deoxy-D-glucose + NAD+
-
 Thermus thermophilus 6-deoxy-D-glucono-delta-lactone + NADH + H+
-
 ?
1.1.1.121 cellobiose + NAD+
-
 Thermus thermophilus ? + NADH + H+
-
 ?
1.1.1.121 cis-benzene dihydrodiol + NAD+
-
 Thermus thermophilus ? + NADH + H+
-
 ?
1.1.1.121 D-fucose + NAD+
-
 Thermus thermophilus D-fucono-delta-lactone + NADH + H+
-
 ?
1.1.1.121 D-galactosamine + NAD+
-
 Thermus thermophilus D-galactosamino-delta-lactone + NADH + H+
-
 ?
1.1.1.121 D-galactose + NAD+
-
 Thermus thermophilus D-galactono-delta-lactone + NADH + H+
-
 ?
1.1.1.121 D-glucosamine + NAD+
-
 Thermus thermophilus D-glucosamino-delta-lactone + NADH + H+
-
 ?
1.1.1.121 D-glucose + NAD+
-
 Thermus thermophilus D-glucono-delta-lactone + NADH + H+
-
 ?
1.1.1.121 D-talose + NAD+
-
 Thermus thermophilus ? + NADH + H+
-
 ?
1.1.1.121 D-xylose + NAD+
-
 Thermus thermophilus D-xylono-delta-lactone + NADH + H+
-
 ?
1.1.1.121 L-arabinose + NAD+
-
 Thermus thermophilus ? + NADH + H+
-
 ?
1.1.1.121 L-fucose + NAD+
-
 Thermus thermophilus ? + NADH + H+
-
 ?
1.1.1.121 lactose + NAD+
-
 Thermus thermophilus ? + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.121 tetramer 4 * 27000, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.121 aldose-1-dehydrogenase
-
 Thermus thermophilus
1.1.1.121 TTHA0369
-
 Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.121 80
-
 mutant enzyme D242N loses about 20% of activity Thermus thermophilus
1.1.1.121 85
-
 15 min, wild-type enzyme is stable, mutant enzyme F141A loses about 5% of activity, mutant enzyme F249A loses about 10% of activity, mutant enzyme D242N loses 60% of activity Thermus thermophilus
1.1.1.121 90
-
 15 min, wild-type enzyme loses about 10% of its activity, mutant enzyme F141A loses about 25% of activity, mutant enzyme F249A loses about 25% of activity, mutant enzyme D242N completely loses activity Thermus thermophilus
1.1.1.121 95
-
 15 min, wild-type enzyme loses about 40% of its activity, mutant enzyme F141A loses about 50% of activity, mutant enzyme F249A loses about 70% of activity, mutant enzyme D242N completely loses activity Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.121 0.075
-
 (R)-tetralol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.11
-
 L-fucose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.17
-
 (R)-indanol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.31
-
 D-galactosamine pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.42
-
 D-xylose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.62
-
 D-glucosamine pH 7.4, 50°C Thermus thermophilus
1.1.1.121 3
-
 D-talose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 3.5
-
 2-Cyclohexen-1-ol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 3.85
-
 D-glucose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 4.55
-
 L-arabinose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 5.77
-
 cellobiose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 6.35
-
 6-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 6.9
-
 2-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 7.4
-
 cis-benzene dihydrodiol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 8.25
-
 D-galactose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 8.7
-
 D-fucose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 9.23
-
 lactose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 12.8
-
 2-deoxy-D-galactose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 40.3
-
 4-chromanol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 138.7
-
 (S)-indanol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 161.7
-
 (S)-tetralol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 4333
-
 1-Acenaphthenol pH 7.4, 50°C Thermus thermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.121 2 13 50°C, 30 min, stable Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.121 NAD+
-
 Thermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.121 0.0005
-
 D-xylose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.00067
-
 L-fucose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.0045
-
 D-glucosamine pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.0067
-
 D-glucose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.012
-
 lactose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.014
-
 D-galactosamine pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.017
-
 L-arabinose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.023
-
 cellobiose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.023
-
 2-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.023
-
 D-talose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.03
-
 (R)-tetralol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.035
-
 6-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.11
-
 D-galactose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.13
-
 (R)-indanol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.27
-
 cis-benzene dihydrodiol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.38
-
 2-deoxy-D-galactose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 0.95
-
 2-Cyclohexen-1-ol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 1.58
-
 D-fucose pH 7.4, 50°C Thermus thermophilus
1.1.1.121 61.7
-
 4-chromanol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 216.7
-
 (S)-indanol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 950
-
 (S)-tetralol pH 7.4, 50°C Thermus thermophilus
1.1.1.121 3000
-
 1-Acenaphthenol pH 7.4, 50°C Thermus thermophilus