Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sun, X.; Lin, Y.; Yuan, Q.; Yan, Y.
    Biological production of muconic acid via a prokaryotic 2,3-dihydroxybenzoic acid decarboxylase (2014), ChemSusChem, 7, 2478-2481 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.1.46 synthesis construction of an artificial pathway for muconic acid production in Escherichia coli by connecting 2,3-dihydroxybenzoic acid biosynthesis with its degradation pathway. Overexpression of entCBA and the key enzymes in the shikimate pathway leads to the production of 900 mg/l of 2,3-dihydroxybenzoic acid. Expression of 2,3-dihydroxybenzoic acid decarboxylase coupled with catechol 1,2-dioxygenase achieves the conversion of 2,3-dihydroxybenzoic acid into muconic acid. Assembly of the total pathway results in the de novo production of muconic acid up to 480 mg/l Klebsiella pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.46 expression in Escherichia coli Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.46 1.42
-
 2,3-Dihydroxybenzoate pH 5.0, 30°C Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.46 Klebsiella pneumoniae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.46 2,3-Dihydroxybenzoate
-
 Klebsiella pneumoniae Catechol + CO2
-
 ?
4.1.1.46 2,4-dihydroxybenzoate
-
 Klebsiella pneumoniae 3-hydroxyphenol + CO2
-
 ?
4.1.1.46 2,5-dihydroxybenzoate
-
 Klebsiella pneumoniae 4-hydroxyphenol + CO2
-
 ?
4.1.1.46 additional information no substrate: 2,4-dihydroxybenzoate Klebsiella pneumoniae ?
-
 ?
4.1.1.46 salicylic acid
-
 Klebsiella pneumoniae phenol + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.46 ? x * 38000, SDS-PAGE, x * 38400, calculated from sequence, recombinant protein with His-tag Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.46 30
-
-
 Klebsiella pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.46 0.065
-
 2,3-Dihydroxybenzoate pH 5.0, 30°C Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.46 5
-
-
 Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.46 additional information no cofactor required Klebsiella pneumoniae