EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.59 | ascorbic acid | 1 mM, 107% of initial activity | Blastobotrys adeninivorans | |
4.1.1.59 | dithiothreitol | 1 mM, 116% of initial activity | Blastobotrys adeninivorans | |
4.1.1.59 | EDTA | 1 mM, 146% of initial activity | Blastobotrys adeninivorans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.59 | Co2+ | 1 mM, 73% loss of activity | Blastobotrys adeninivorans | |
4.1.1.59 | Cu2+ | 1 mM, 96% loss of activity | Blastobotrys adeninivorans | |
4.1.1.59 | Fe2+ | 1 mM, complete loss of activity | Blastobotrys adeninivorans | |
4.1.1.59 | Fe3+ | 1 mM, 76% loss of activity | Blastobotrys adeninivorans | |
4.1.1.59 | Ni2+ | 1 mM, complete loss of activity | Blastobotrys adeninivorans | |
4.1.1.59 | thiosulfate | 1 mM, 15% loss of activity | Blastobotrys adeninivorans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.59 | 0.7 | - |
gallic acid | pH 6.2, 40°C | Blastobotrys adeninivorans | |
4.1.1.59 | 3.2 | - |
protocatechuic acid | pH 6.2, 40°C | Blastobotrys adeninivorans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.59 | 27100 | - |
gel filtration | Blastobotrys adeninivorans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.59 | Blastobotrys adeninivorans | A0A060TAG5 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.1.59 | 5.9 | - |
pH 6.2, 40°C | Blastobotrys adeninivorans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.59 | gallic acid | - |
Blastobotrys adeninivorans | pyrogallol + CO2 | - |
? | |
4.1.1.59 | additional information | no substrtaes: 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, 2,3-dihydroxybenzoic acid, 2,4-dihydroxybenzoic acid and 2,5-dihydroxybenzoic acid | Blastobotrys adeninivorans | ? | - |
? | |
4.1.1.59 | protocatechuic acid | - |
Blastobotrys adeninivorans | catechol + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.59 | ? | x * 27300, calculated from sequence, x * 25000, SDS-PAGE, recombinant protein | Blastobotrys adeninivorans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.59 | 40 | - |
- |
Blastobotrys adeninivorans |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.59 | 25 | 45 | more than 80% of maximum activity | Blastobotrys adeninivorans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.59 | 4 | 30 | stable within | Blastobotrys adeninivorans |
4.1.1.59 | 35 | - |
24 h, 60% loss of activity | Blastobotrys adeninivorans |
4.1.1.59 | 40 | - |
stable for 7 h, rapid loss of activity thereafter | Blastobotrys adeninivorans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.59 | 42 | - |
gallic acid | pH 6.2, 40°C | Blastobotrys adeninivorans | |
4.1.1.59 | 44 | - |
protocatechuic acid | pH 6.2, 40°C | Blastobotrys adeninivorans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.59 | 6.2 | - |
- |
Blastobotrys adeninivorans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.59 | 5.6 | 7.1 | more than 80% of maximum activity | Blastobotrys adeninivorans |
EC Number | Organism | Comment | Expression |
---|---|---|---|
4.1.1.59 | Blastobotrys adeninivorans | expression is induced by gallic acid and protocatechuic acid | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.1.59 | physiological function | a gene deletion mutant is not able to grow on medium with gallic acid as carbon source but can grow in presence of protocatechuic acid | Blastobotrys adeninivorans |