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Literature summary extracted from

  • Meier, A.; Worch, S.; Boer, E.; Hartmann, A.; Mascher, M.; Marzec, M.; Scholz, U.; Riechen, J.; Baronian, K.; Schauer, F.; Bode, R.; Kunze, G.
    Agdc1p - a gallic acid decarboxylase involved in the degradation of tannic acid in the yeast Blastobotrys (Arxula) adeninivorans (2017), Front. Microbiol., 8, 1777 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.59 ascorbic acid 1 mM, 107% of initial activity Blastobotrys adeninivorans
4.1.1.59 dithiothreitol 1 mM, 116% of initial activity Blastobotrys adeninivorans
4.1.1.59 EDTA 1 mM, 146% of initial activity Blastobotrys adeninivorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.59 Co2+ 1 mM, 73% loss of activity Blastobotrys adeninivorans
4.1.1.59 Cu2+ 1 mM, 96% loss of activity Blastobotrys adeninivorans
4.1.1.59 Fe2+ 1 mM, complete loss of activity Blastobotrys adeninivorans
4.1.1.59 Fe3+ 1 mM, 76% loss of activity Blastobotrys adeninivorans
4.1.1.59 Ni2+ 1 mM, complete loss of activity Blastobotrys adeninivorans
4.1.1.59 thiosulfate 1 mM, 15% loss of activity Blastobotrys adeninivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.59 0.7
-
gallic acid pH 6.2, 40°C Blastobotrys adeninivorans
4.1.1.59 3.2
-
protocatechuic acid pH 6.2, 40°C Blastobotrys adeninivorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.59 27100
-
gel filtration Blastobotrys adeninivorans

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.59 Blastobotrys adeninivorans A0A060TAG5
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.59 5.9
-
pH 6.2, 40°C Blastobotrys adeninivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.59 gallic acid
-
Blastobotrys adeninivorans pyrogallol + CO2
-
?
4.1.1.59 additional information no substrtaes: 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, 2,3-dihydroxybenzoic acid, 2,4-dihydroxybenzoic acid and 2,5-dihydroxybenzoic acid Blastobotrys adeninivorans ?
-
?
4.1.1.59 protocatechuic acid
-
Blastobotrys adeninivorans catechol + CO2
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.59 ? x * 27300, calculated from sequence, x * 25000, SDS-PAGE, recombinant protein Blastobotrys adeninivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.59 40
-
-
Blastobotrys adeninivorans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.59 25 45 more than 80% of maximum activity Blastobotrys adeninivorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.59 4 30 stable within Blastobotrys adeninivorans
4.1.1.59 35
-
24 h, 60% loss of activity Blastobotrys adeninivorans
4.1.1.59 40
-
stable for 7 h, rapid loss of activity thereafter Blastobotrys adeninivorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.59 42
-
gallic acid pH 6.2, 40°C Blastobotrys adeninivorans
4.1.1.59 44
-
protocatechuic acid pH 6.2, 40°C Blastobotrys adeninivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.59 6.2
-
-
Blastobotrys adeninivorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.59 5.6 7.1 more than 80% of maximum activity Blastobotrys adeninivorans

Expression

EC Number Organism Comment Expression
4.1.1.59 Blastobotrys adeninivorans expression is induced by gallic acid and protocatechuic acid up

General Information

EC Number General Information Comment Organism
4.1.1.59 physiological function a gene deletion mutant is not able to grow on medium with gallic acid as carbon source but can grow in presence of protocatechuic acid Blastobotrys adeninivorans