BRENDA - Enzyme Database

Glutamine hydrolysis by imidazole glycerol phosphate synthase displays temperature dependent allosteric activation

Lisi, G.; Currier, A.; Loria, J.; Front. Mol. Biosci. 5, 4 (2018)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.3.2.10
N'-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide-ribonucleotide
binding of the allosteric effector ligand stimulates millisecond timescale motions in the enzyme that enhance its catalytic function. The flexibility of the apo enzyme is nearly identical to that of its N'-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide-ribonucleotide activated state at 70C, whereas conformational motions are considerably different between the two forms of the enzyme at room temperature. Allosteric activation by N'-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide-ribonucleotide decreases to 65fold at 70C, compared to 4200fold at 30C
Thermotoga maritima
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
4.3.2.10
-
Thermotoga maritima
4.3.2.10
expression in Escherichia coli BL21(DE3)
Thermotoga maritima
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.2.10
1.3
-
L-glutamine
pH 8.0, 30C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
1.32
-
L-glutamine
pH 8.0, 70C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
1.35
-
L-glutamine
pH 8.0, 40C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
1.36
-
L-glutamine
pH 8.0, 60C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
1.64
-
L-glutamine
pH 8.0, 50C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
2.96
-
L-glutamine
pH 8.0, 70C
Thermotoga maritima
4.3.2.10
2.96
-
L-glutamine
pH 8.0, 70C, basal activity
Thermotoga maritima
4.3.2.10
3.8
-
L-glutamine
pH 8.0, 50C
Thermotoga maritima
4.3.2.10
3.8
-
L-glutamine
pH 8.0, 50C, basal activity
Thermotoga maritima
4.3.2.10
3.89
-
L-glutamine
pH 8.0, 60C
Thermotoga maritima
4.3.2.10
3.89
-
L-glutamine
pH 8.0, 60C, basal activity
Thermotoga maritima
4.3.2.10
4.71
-
L-glutamine
pH 8.0, 40C
Thermotoga maritima
4.3.2.10
4.71
-
L-glutamine
pH 8.0, 40C, basal activity
Thermotoga maritima
4.3.2.10
4.91
-
L-glutamine
pH 8.0, 30C
Thermotoga maritima
4.3.2.10
4.91
-
L-glutamine
pH 8.0, 30C, basal activity
Thermotoga maritima
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
Thermotoga maritima
the enzyme is involved in histidine biosynthesis
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
Thermotoga maritima ATCC 43589
the enzyme is involved in histidine biosynthesis
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
4.3.2.10
Thermotoga maritima
Q9X0C6 AND Q9X0C8
Q9X0C6: subunit HisF, Q9X0C8: subunit HisH
-
4.3.2.10
Thermotoga maritima
Q9X0C8 and Q9X0C6
Q9X0C8: subunit HisH, Q9X0C6: subunit HisF
-
4.3.2.10
Thermotoga maritima ATCC 43589
Q9X0C6 AND Q9X0C8
Q9X0C6: subunit HisF, Q9X0C8: subunit HisH
-
4.3.2.10
Thermotoga maritima ATCC 43589
Q9X0C8 and Q9X0C6
Q9X0C8: subunit HisH, Q9X0C6: subunit HisF
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
4.3.2.10
-
Thermotoga maritima
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
-
747861
Thermotoga maritima
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
the enzyme is involved in histidine biosynthesis
747861
Thermotoga maritima
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
-
747861
Thermotoga maritima ATCC 43589
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
the enzyme is involved in histidine biosynthesis
747861
Thermotoga maritima ATCC 43589
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
4.3.2.10
L-glutamine + H2O
-
747861
Thermotoga maritima
L-glutamate + NH3
-
-
-
?
4.3.2.10
L-glutamine + H2O
binding of the allosteric effector ligand 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide stimulates millisecond timescale motions in IGPS that enhance its catalytic function. Allosteric activation decreases to 65-fold at 70C, compared to 4200-fold at 30C
747861
Thermotoga maritima
L-glutamate + NH3
-
-
-
?
4.3.2.10
L-glutamine + H2O
binding of the allosteric effector ligand 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide stimulates millisecond timescale motions in IGPS that enhance its catalytic function. Allosteric activation decreases to 65-fold at 70C, compared to 4200-fold at 30C
747861
Thermotoga maritima ATCC 43589
L-glutamate + NH3
-
-
-
?
4.3.2.10
L-glutamine + H2O
-
747861
Thermotoga maritima ATCC 43589
L-glutamate + NH3
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
4.3.2.10
gps
-
Thermotoga maritima
4.3.2.10
IGPS
-
Thermotoga maritima
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.3.2.10
30
-
assay at
Thermotoga maritima
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
4.3.2.10
30
70
binding of the allosteric effector ligand 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide stimulates millisecond timescale motions in IGPS that enhance its catalytic function. Allosteric activation decreases to 65-fold at 70C, compared to 4200-fold at 30C
Thermotoga maritima
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.3.2.10
0.00372
-
L-glutamine
pH 8.0, 30C
Thermotoga maritima
4.3.2.10
0.00372
-
L-glutamine
pH 8.0, 30C, basal activity
Thermotoga maritima
4.3.2.10
0.0571
-
L-glutamine
pH 8.0, 40C
Thermotoga maritima
4.3.2.10
0.0571
-
L-glutamine
pH 8.0, 40C, basal activity
Thermotoga maritima
4.3.2.10
0.139
-
L-glutamine
pH 8.0, 50C
Thermotoga maritima
4.3.2.10
0.139
-
L-glutamine
pH 8.0, 50C, basal activity
Thermotoga maritima
4.3.2.10
0.204
-
L-glutamine
pH 8.0, 70C
Thermotoga maritima
4.3.2.10
0.204
-
L-glutamine
pH 8.0, 70C, basal activity
Thermotoga maritima
4.3.2.10
0.212
-
L-glutamine
pH 8.0, 60C
Thermotoga maritima
4.3.2.10
0.212
-
L-glutamine
pH 8.0, 60C, basal activity
Thermotoga maritima
4.3.2.10
4.09
-
L-glutamine
pH 8.0, 30C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
4.37
-
L-glutamine
pH 8.0, 40C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
4.89
-
L-glutamine
pH 8.0, 50C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
5.5
-
L-glutamine
pH 8.0, 60C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
5.92
-
L-glutamine
pH 8.0, 70C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.2.10
8
-
assay at
Thermotoga maritima
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.3.2.10
N'-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide-ribonucleotide
binding of the allosteric effector ligand stimulates millisecond timescale motions in the enzyme that enhance its catalytic function. The flexibility of the apo enzyme is nearly identical to that of its N'-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide-ribonucleotide activated state at 70C, whereas conformational motions are considerably different between the two forms of the enzyme at room temperature. Allosteric activation by N'-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide-ribonucleotide decreases to 65fold at 70C, compared to 4200fold at 30C
Thermotoga maritima
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.2.10
-
Thermotoga maritima
4.3.2.10
expression in Escherichia coli BL21(DE3)
Thermotoga maritima
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.2.10
1.3
-
L-glutamine
pH 8.0, 30C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
1.32
-
L-glutamine
pH 8.0, 70C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
1.35
-
L-glutamine
pH 8.0, 40C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
1.36
-
L-glutamine
pH 8.0, 60C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
1.64
-
L-glutamine
pH 8.0, 50C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
2.96
-
L-glutamine
pH 8.0, 70C
Thermotoga maritima
4.3.2.10
2.96
-
L-glutamine
pH 8.0, 70C, basal activity
Thermotoga maritima
4.3.2.10
3.8
-
L-glutamine
pH 8.0, 50C
Thermotoga maritima
4.3.2.10
3.8
-
L-glutamine
pH 8.0, 50C, basal activity
Thermotoga maritima
4.3.2.10
3.89
-
L-glutamine
pH 8.0, 60C
Thermotoga maritima
4.3.2.10
3.89
-
L-glutamine
pH 8.0, 60C, basal activity
Thermotoga maritima
4.3.2.10
4.71
-
L-glutamine
pH 8.0, 40C
Thermotoga maritima
4.3.2.10
4.71
-
L-glutamine
pH 8.0, 40C, basal activity
Thermotoga maritima
4.3.2.10
4.91
-
L-glutamine
pH 8.0, 30C
Thermotoga maritima
4.3.2.10
4.91
-
L-glutamine
pH 8.0, 30C, basal activity
Thermotoga maritima
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
Thermotoga maritima
the enzyme is involved in histidine biosynthesis
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
Thermotoga maritima ATCC 43589
the enzyme is involved in histidine biosynthesis
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.2.10
-
Thermotoga maritima
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
-
747861
Thermotoga maritima
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
the enzyme is involved in histidine biosynthesis
747861
Thermotoga maritima
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
-
747861
Thermotoga maritima ATCC 43589
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
the enzyme is involved in histidine biosynthesis
747861
Thermotoga maritima ATCC 43589
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
4.3.2.10
L-glutamine + H2O
-
747861
Thermotoga maritima
L-glutamate + NH3
-
-
-
?
4.3.2.10
L-glutamine + H2O
binding of the allosteric effector ligand 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide stimulates millisecond timescale motions in IGPS that enhance its catalytic function. Allosteric activation decreases to 65-fold at 70C, compared to 4200-fold at 30C
747861
Thermotoga maritima
L-glutamate + NH3
-
-
-
?
4.3.2.10
L-glutamine + H2O
binding of the allosteric effector ligand 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide stimulates millisecond timescale motions in IGPS that enhance its catalytic function. Allosteric activation decreases to 65-fold at 70C, compared to 4200-fold at 30C
747861
Thermotoga maritima ATCC 43589
L-glutamate + NH3
-
-
-
?
4.3.2.10
L-glutamine + H2O
-
747861
Thermotoga maritima ATCC 43589
L-glutamate + NH3
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.3.2.10
30
-
assay at
Thermotoga maritima
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
4.3.2.10
30
70
binding of the allosteric effector ligand 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide stimulates millisecond timescale motions in IGPS that enhance its catalytic function. Allosteric activation decreases to 65-fold at 70C, compared to 4200-fold at 30C
Thermotoga maritima
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.3.2.10
0.00372
-
L-glutamine
pH 8.0, 30C
Thermotoga maritima
4.3.2.10
0.00372
-
L-glutamine
pH 8.0, 30C, basal activity
Thermotoga maritima
4.3.2.10
0.0571
-
L-glutamine
pH 8.0, 40C
Thermotoga maritima
4.3.2.10
0.0571
-
L-glutamine
pH 8.0, 40C, basal activity
Thermotoga maritima
4.3.2.10
0.139
-
L-glutamine
pH 8.0, 50C
Thermotoga maritima
4.3.2.10
0.139
-
L-glutamine
pH 8.0, 50C, basal activity
Thermotoga maritima
4.3.2.10
0.204
-
L-glutamine
pH 8.0, 70C
Thermotoga maritima
4.3.2.10
0.204
-
L-glutamine
pH 8.0, 70C, basal activity
Thermotoga maritima
4.3.2.10
0.212
-
L-glutamine
pH 8.0, 60C
Thermotoga maritima
4.3.2.10
0.212
-
L-glutamine
pH 8.0, 60C, basal activity
Thermotoga maritima
4.3.2.10
4.09
-
L-glutamine
pH 8.0, 30C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
4.37
-
L-glutamine
pH 8.0, 40C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
4.89
-
L-glutamine
pH 8.0, 50C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
5.5
-
L-glutamine
pH 8.0, 60C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
5.92
-
L-glutamine
pH 8.0, 70C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.2.10
8
-
assay at
Thermotoga maritima
General Information
EC Number
General Information
Commentary
Organism
4.3.2.10
metabolism
the enzyme is involved in histidine biosynthesis
Thermotoga maritima
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.3.2.10
metabolism
the enzyme is involved in histidine biosynthesis
Thermotoga maritima
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.3.2.10
0.000758
-
L-glutamine
pH 8.0, 30C
Thermotoga maritima
4.3.2.10
0.0121
-
L-glutamine
pH 8.0, 40C
Thermotoga maritima
4.3.2.10
0.0365
-
L-glutamine
pH 8.0, 50C
Thermotoga maritima
4.3.2.10
0.054
-
L-glutamine
pH 8.0, 60C
Thermotoga maritima
4.3.2.10
0.0689
-
L-glutamine
pH 8.0, 70C
Thermotoga maritima
4.3.2.10
0.121
-
L-glutamine
pH 8.0, 40C, basal activity
Thermotoga maritima
4.3.2.10
0.365
-
L-glutamine
pH 8.0, 50C, basal activity
Thermotoga maritima
4.3.2.10
0.544
-
L-glutamine
pH 8.0, 60C, basal activity
Thermotoga maritima
4.3.2.10
0.688
-
L-glutamine
pH 8.0, 70C, basal activity
Thermotoga maritima
4.3.2.10
0.758
-
L-glutamine
pH 8.0, 30C, basal activity
Thermotoga maritima
4.3.2.10
2980
-
L-glutamine
pH 8.0, 50C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
3150
-
L-glutamine
pH 8.0, 30C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
3240
-
L-glutamine
pH 8.0, 40C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
4040
-
L-glutamine
pH 8.0, 60C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
4480
-
L-glutamine
pH 8.0, 70C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.3.2.10
0.000758
-
L-glutamine
pH 8.0, 30C
Thermotoga maritima
4.3.2.10
0.0121
-
L-glutamine
pH 8.0, 40C
Thermotoga maritima
4.3.2.10
0.0365
-
L-glutamine
pH 8.0, 50C
Thermotoga maritima
4.3.2.10
0.054
-
L-glutamine
pH 8.0, 60C
Thermotoga maritima
4.3.2.10
0.0689
-
L-glutamine
pH 8.0, 70C
Thermotoga maritima
4.3.2.10
0.121
-
L-glutamine
pH 8.0, 40C, basal activity
Thermotoga maritima
4.3.2.10
0.365
-
L-glutamine
pH 8.0, 50C, basal activity
Thermotoga maritima
4.3.2.10
0.544
-
L-glutamine
pH 8.0, 60C, basal activity
Thermotoga maritima
4.3.2.10
0.688
-
L-glutamine
pH 8.0, 70C, basal activity
Thermotoga maritima
4.3.2.10
0.758
-
L-glutamine
pH 8.0, 30C, basal activity
Thermotoga maritima
4.3.2.10
2980
-
L-glutamine
pH 8.0, 50C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
3150
-
L-glutamine
pH 8.0, 30C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
3240
-
L-glutamine
pH 8.0, 40C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
4040
-
L-glutamine
pH 8.0, 60C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima
4.3.2.10
4480
-
L-glutamine
pH 8.0, 70C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Thermotoga maritima