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Literature summary extracted from

  • Xavier, K.; Peist, R.; Kossmann, M.; Boos, W.; Santos, H.
    Maltose metabolism in the hyperthermophilic archaeon Thermococcus litoralis Purification and characterization of key enzymes (1999), J. Bacteriol., 181, 3358-3367 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25 expression in Escherichia coli Thermococcus litoralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.25 0.46
-
 maltoheptaose pH 7.0, 95°C Thermococcus litoralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.25 134000
-
 gel filtration Thermococcus litoralis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Thermococcus litoralis O32462 DSM 5473
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25
-
 Thermococcus litoralis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.25 25.7
-
 substrate: maltotriose, pH 7.0, 95°C Thermococcus litoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 maltoheptaose
-
 Thermococcus litoralis maltodextrin + D-glucose
-
 ?
2.4.1.25 maltose + D-glucose
-
 Thermococcus litoralis maltodextrin + D-glucose
-
 ?
2.4.1.25 maltotriose
-
 Thermococcus litoralis maltodextrin + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.25 dimer 2 * 79000, SDS-PAGE Thermococcus litoralis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.25 4-alpha-glucanotransferase
-
 Thermococcus litoralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 95
-
-
 Thermococcus litoralis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.25 70 105 70°C: about 60% of maximal activity, 105°C: about 50% of maximal activity Thermococcus litoralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 7
-
 assay at Thermococcus litoralis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.25 Thermococcus litoralis isoelectric focusing
-
 3.5

Expression

EC Number Organism Comment Expression
2.4.1.25 Thermococcus litoralis the enzyme is constitutive additional information

General Information

EC Number General Information Comment Organism
2.4.1.25 metabolism key enzymes in the pathway for maltose catabolism. Glucose derived from the action of 4-alpha-glucanotransferase is subsequently metabolized via an Embden-Meyerhof pathway Thermococcus litoralis