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Literature summary extracted from

  • Yu, H.; Zhao, S.; Guo, L.
    Novel Gene encoding 5-aminosalicylate 1,2-dioxygenase from Comamonas sp. strain QT12 and catalytic properties of the purified enzyme (2018), J. Bacteriol., 200, e00395-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.86 heterologously expressed in Escherichia coli as a His-tagged enzyme Comamonas thiooxydans

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.86 G93A the mutant enzyme shows the ability to oxidize salicylate, the wild-type enzyme shows no activity with salicylate Comamonas thiooxydans
1.13.11.86 H107A mutant enzyme shows no enzymatic activity Comamonas thiooxydans
1.13.11.86 H109A mutant enzyme shows no enzymatic activity Comamonas thiooxydans
1.13.11.86 H150A mutant enzyme shows no enzymatic activity Comamonas thiooxydans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.86 1,10-phenanthroline 0.01 mM, 89% inhibition Comamonas thiooxydans
1.13.11.86 EDTA 1 mM, 31% inhibition Comamonas thiooxydans
1.13.11.86 SDS 1 mM, complete inhibition Comamonas thiooxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.86 0.052
-
 5-Aminosalicylate pH 8.0, 25°C, wild-type enzyme Comamonas thiooxydans
1.13.11.86 0.823
-
 gentisate pH 8.0, 25°C, wild-type enzyme Comamonas thiooxydans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.86 Fe2+ required for the catalytic activity of the purified enzyme, nonheme iron dioxygenase. The enzyme activity of MabB increases about 2.3fold with the addition of 0.25 mM Fe2+, and 74% enzyme activity is observed when 0.25 mM Fe3+ is added Comamonas thiooxydans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.86 45000
-
 SDS-PAGE Comamonas thiooxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.86 5-aminosalicylate + O2 Comamonas thiooxydans the enzyme plays an important physiological role in 3-aminobenzoate degradation in Comamonas sp. strain QT12 (2Z,4E)-4-amino-6-oxohepta-2,4-dienedioate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.86 Comamonas thiooxydans A0A1V0ELS9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.86
-
 Comamonas thiooxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.86 1-hydroxy-2-naphthoate + O2 oxidized by MabB at a rate less than 1% of that observed for 5-aminosalicylate Comamonas thiooxydans (3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
-
 ?
1.13.11.86 5-aminosalicylate + O2 the enzyme plays an important physiological role in 3-aminobenzoate degradation in Comamonas sp. strain QT12 Comamonas thiooxydans (2Z,4E)-4-amino-6-oxohepta-2,4-dienedioate
-
 ?
1.13.11.86 5-aminosalicylate + O2 the two oxygen atoms incorporated into the product are both from the dioxygen molecule. Both 5-aminosalicylate and gentisate can be converted by MabB. The catalytic efficiency of MabB for 5-aminosalicylate is much higher (about 70fold) than that for gentisate Comamonas thiooxydans (2Z,4E)-4-amino-6-oxohepta-2,4-dienedioate
-
 ?
1.13.11.86 gentisate + O2 the two oxygen atoms incorporated into the product are both from the dioxygen molecule. Both 5-aminosalicylate and gentisate can be converted by MabB. The catalytic efficiency of MabB for 5-aminosalicylate is much higher (about 70fold) than that for gentisate Comamonas thiooxydans maleylpyruvate
-
 ?
1.13.11.86 additional information no activity is detected in reaction with salicylate Comamonas thiooxydans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.86 ? x * 45000, SDS-PAGE Comamonas thiooxydans

Synonyms

EC Number Synonyms Comment Organism
1.13.11.86 mabB
-
 Comamonas thiooxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.86 10
-
-
 Comamonas thiooxydans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.86 10 45 10°C: maximal activity, 45°C: about 60% of maximal activity Comamonas thiooxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.86 7.5 8
-
 Comamonas thiooxydans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.86 6.5 9 pH 6.5: about 50% of maximal activity, pH 9.0: about 40% of maximal activity Comamonas thiooxydans

Expression

EC Number Organism Comment Expression
1.13.11.86 Comamonas thiooxydans the enzyme is induced by 3-aminobenzoate or its degradation intermediates up

General Information

EC Number General Information Comment Organism
1.13.11.86 malfunction the mabB-disrupted mutant loses the ability to grow on 3-aminobenzoate Comamonas thiooxydans
1.13.11.86 metabolism the enzyme plays an important physiological role in 3-aminobenzoate degradation in Comamonas sp. strain QT12 Comamonas thiooxydans