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Literature summary extracted from

  • Lipchock, J.; Loria, J.P.
    Millisecond dynamics in the allosteric enzyme imidazole glycerol phosphate synthase (IGPS) from Thermotoga maritima (2009), J. Biomol. NMR, 45, 73-84 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.10
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 Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.10 Thermotoga maritima Q9X0C6 AND Q9X0C8 Q9X0C6: subunit HisF, Q9X0C8: subunit HisH
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4.3.2.10 Thermotoga maritima ATCC 43589 Q9X0C6 AND Q9X0C8 Q9X0C6: subunit HisF, Q9X0C8: subunit HisH
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Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.10
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine IGPS heterodimeric enzyme comprised of two proteins, HisH and HisF, that catalyze the hydrolysis of glutamine to produce NH3 in the HisH active site and the cyclization of ammonia with 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide in HisF to produce 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide and D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate. Binding of 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide and D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate stimulates glutaminase activity in the HisH enzyme over 5000 and 100fold, respectively, despite the active sites being more than 25 A apart Thermotoga maritima 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
 ?
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine IGPS heterodimeric enzyme comprised of two proteins, HisH and HisF, that catalyze the hydrolysis of glutamine to produce NH3 in the HisH active site and the cyclization of ammonia with 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide in HisF to produce 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide and D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate. Binding of 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide and D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate stimulates glutaminase activity in the HisH enzyme over 5000 and 100fold, respectively, despite the active sites being more than 25 A apart Thermotoga maritima ATCC 43589 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
 ?
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-ß-D-ribosyl)imidazole-4-carboxamide + NH3 IGPS heterodimeric enzyme comprised of two proteins, HisH and HisF, that catalyze the hydrolysis of glutamine to produce NH3 in the HisH active site and the cyclization of ammonia with 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide in HisF to produce 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide and D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate. Binding of 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide and D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate stimulates glutaminase activity in the HisH enzyme over 5000 and 100-fold, respectively, despite the active sites being more than 25 A apart Thermotoga maritima 5-amino-1-(5-phospho-ß-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H2O
-
 ?
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-ß-D-ribosyl)imidazole-4-carboxamide + NH3 IGPS heterodimeric enzyme comprised of two proteins, HisH and HisF, that catalyze the hydrolysis of glutamine to produce NH3 in the HisH active site and the cyclization of ammonia with 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide in HisF to produce 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide and D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate. Binding of 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide and D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate stimulates glutaminase activity in the HisH enzyme over 5000 and 100-fold, respectively, despite the active sites being more than 25 A apart Thermotoga maritima ATCC 43589 5-amino-1-(5-phospho-ß-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H2O
-
 ?
4.3.2.10 L-glutamine + H2O IGPS heterodimeric enzyme comprised of two proteins, HisH and HisF, that catalyze the hydrolysis of glutamine to produce NH3 in the HisH active site and the cyclization of ammonia with 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide in HisF to produce 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide and D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate. Binding of 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide and D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate stimulates glutaminase activity in the HisH enzyme over 5000- and 100fold, respectively, despite the active sites being more than 25 A apart Thermotoga maritima L-glutamate + NH3
-
 ?
4.3.2.10 L-glutamine + H2O IGPS heterodimeric enzyme comprised of two proteins, HisH and HisF, that catalyze the hydrolysis of glutamine to produce NH3 in the HisH active site and the cyclization of ammonia with 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide in HisF to produce 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide and D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate. Binding of 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide and D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate stimulates glutaminase activity in the HisH enzyme over 5000- and 100fold, respectively, despite the active sites being more than 25 A apart Thermotoga maritima ATCC 43589 L-glutamate + NH3
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 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.2.10 IGPS
-
 Thermotoga maritima