Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Iwai, M.; Yamada, H.; Ikemoto, T.; Matsumoto, S.; Fujiwara, D.; Takenaka, S.; Sakamoto, T.
    Biochemical characterization and overexpression of an endo-rhamnogalacturonan lyase from Penicillium chrysogenum (2015), Mol. Biotechnol., 57, 539-548 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.23 expression in Escherichia coli Penicillium chrysogenum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.23 Cu2+ 10% of initial activity Penicillium chrysogenum
4.2.2.23 Hg2+ 3% of initial activity Penicillium chrysogenum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.23 additional information
-
 degalactosylated rhamnogalacturonan Km value 0.74 mg/ml, pH 7.0, 37°C Penicillium chrysogenum
4.2.2.23 additional information
-
 rhamnogalacturonan Km value 1.67 mg/ml, pH 7.0, 37°C Penicillium chrysogenum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.23 extracellular
-
 Penicillium chrysogenum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.23 Ca2+ 1 mM, 130% of initial activity Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.23 Penicillium chrysogenum W8VJU1
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.2.23 no glycoprotein protein does not contain N-linked carbohydrates Penicillium chrysogenum

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.23 from culture liquid Penicillium chrysogenum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.23 culture fluid
-
 Penicillium chrysogenum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.23 2 2.5 substrate rhamnogalacturonan, pH 7.0, 37°C Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.23 azurine-crosslinked rhamnogalacturonan
-
 Penicillium chrysogenum azurine-crosslinked unsaturated rhamnogalacturonan oligosaccharides
-
 ?
4.2.2.23 degalactosylated rhamnogalacturonan
-
 Penicillium chrysogenum ?
-
 ?
4.2.2.23 additional information no substrate: homogalacturonan Penicillium chrysogenum ?
-
 ?
4.2.2.23 rhamnogalacturonan i.e. soybean rhamnogalacturonan Penicillium chrysogenum rhamnogalacturonan tetrasaccharide with 4-deoxy-4,5-unsaturated galacturonic acid at the nonreducing end
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.23 ? x * 51000, SDS-PAGE, x * 54449, calculated from sequence Penicillium chrysogenum

Synonyms

EC Number Synonyms Comment Organism
4.2.2.23 Rgl4A
-
 Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.23 40
-
-
 Penicillium chrysogenum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.23 40
-
 pH 7.0, stable for at least 1 h Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.23 7 8
-
 Penicillium chrysogenum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.23 7
-
 40°C, stable for at least 1 h Penicillium chrysogenum