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Literature summary extracted from

  • Rajniak, J.; Giehl, R.; Chang, E.; Murgia, I.; Von Wiren, N.; Sattely, E.
    Biosynthesis of redox-active metabolites in response to iron deficiency in plants (2018), Nat. Chem. Biol., 14, 442-450 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.60 heterologous-pathway reconstitution in Nicotiana benthamiana Arabidopsis thaliana
1.14.14.164 heterologous-pathway reconstitution in Nicotiana benthamiana Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.60 scopoletin + 2-oxoglutarate + O2 Arabidopsis thaliana the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants fraxetin + succinate + CO2
-
 ?
1.14.14.164 fraxetin + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants sideretin (reduced form) + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.60 Arabidopsis thaliana Q9LE86
-
-
1.14.14.164 Arabidopsis thaliana Q9SZ46
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.164
-
 Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.60 root expressed most strongly in the root epidermal layer under iron-deficient conditions Arabidopsis thaliana
-
1.14.14.164 root expressed most strongly in the root epidermal layer under iron-deficient conditions Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.60 scopoletin + 2-oxoglutarate + O2
-
 Arabidopsis thaliana fraxetin + succinate + CO2
-
 ?
1.14.11.60 scopoletin + 2-oxoglutarate + O2 the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants Arabidopsis thaliana fraxetin + succinate + CO2
-
 ?
1.14.14.164 8-methoxypsoralen + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana 5-hydroxy-8-methoxypsoralen + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.164 fraxetin + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana sideretin (reduced form) + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.164 fraxetin + [reduced NADPH-hemoprotein reductase] + O2 the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants Arabidopsis thaliana sideretin (reduced form) + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.60 At3g12900
-
 Arabidopsis thaliana
1.14.11.60 S8H
-
 Arabidopsis thaliana
1.14.14.164 At4g31940
-
 Arabidopsis thaliana
1.14.14.164 CYP82C4
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.164 cytochrome P-450 a cytochrome P-450 (heme thiolate) enzyme Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
1.14.11.60 Arabidopsis thaliana iron-deficiency-dependent upregulation. The iron-deficiency-dependent upregulation is mainly confined to roots up
1.14.14.164 Arabidopsis thaliana expression is upregulated by iron deficiency. The iron-deficiency-dependent upregulation is mainly confined to roots up

General Information

EC Number General Information Comment Organism
1.14.11.60 metabolism the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants. Under iron-deficiency conditions, a branch of the phenylpropanoid pathway consisting of feruloyl-CoA 6'-hydroxylase 1, scopoletin 8-hydroxylase, and CYP82C4 is strongly induced, thus leading to the synthesis of scopoletin and its conversion into redox-active fraxetin and sideretin. In the rhizosphere, the redox-active coumarins act in concert with other components of the strategy I iron acquisition machinery by solubilizing and reducing iron from sparingly available sources Arabidopsis thaliana
1.14.14.164 metabolism the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants. Under iron-deficiency conditions, a branch of the phenylpropanoid pathway consisting of feruloyl-CoA 6'-hydroxylase 1, scopoletin 8-hydroxylase, and CYP82C4 is strongly induced, thus leading to the synthesis of scopoletin and its conversion into redox-active fraxetin and sideretin. In the rhizosphere, the redox-active coumarins act in concert with other components of the strategy I iron acquisition machinery by solubilizing and reducing iron from sparingly available sources Arabidopsis thaliana